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Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmus maximus, against pathogenic vibrio ArchiMer
Gatesoupe, Fj.
Characteristics of Lactic Acid Bacteria (LAB) isolated from rotifers, Brachionus plicatilis, resembled those of Lactobacillus plantarum or Carnobacterium sp. It was investigated whether the artificial maintenance of a high concentration of LAB in rotifers might increase their dietary value for turbot larvae, particularly when the fish were infected with pathogenic Vibrio sp. One LAB strain was cultivated, then introduced daily into the enrichment medium of rotifers. In this way, LAB were retrieved in large amounts in turbot, and a significant limitation of larval mortality rate was observed when turbot were challenged with pathogenic vibrio at day 9. The inoculum concentration of LAB had a decisive effect on survival rate, and the optimum was between 10(7)...
Tipo: Text Palavras-chave: LACTIC ACID BACTERIA; VIBRIO; PATHOGEN; ROTIFER; EXPERIMENTAL INFECTION; LARVAL FISH CULTURE; TURBOT; PLEURONECTIFORMES.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00065/17672/15200.pdf
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Siderophore production and probiotic effect of Vibrio sp. associated with turbot larvae, Scophthalmus maximus ArchiMer
Gatesoupe, Fj.
The proportion of vibrio E, dominant in healthy turbot larvae, was artificially increased in the rotifer enrichment medium. The maid effect of this vibrio enrichment was to improve the resistance of larval turbot challenged with a pathogenic strain of Vibrio splendidus, vibrio P. The improvement of the survival rates was significant with 1.5 x 10(6) CFU of vibrio E added per mi of enrichment medium, and it was still maximum at the hi hest dose tested (5 x 10(7) CFU.ml(-1); 80 +/- 2% of survivors after 48 h of challenge, versus 50 +/- 2 % for the control group). Further in vitro characterisation showed that vibrio E was able to grow in the presence of the chelating agents EDDHA and EDTA, while producing siderophore, as revealed by chrome azurol S dyeing....
Tipo: Text Palavras-chave: Bacteria; Pathogenic Vibrio; Probiotic Vibrio; Experimental infection; Siderophore; Turbot; Larva.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00065/17671/15199.pdf
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