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Monoclonal antibody against the surface of Alexandrium minutum used in a whole-cell ELISA ArchiMer
Gas, F; Pinto, L; Baus, B; Gaufres, L; Crassous, Marie-pierre; Compere, Chantal; Quemeneur, E.
Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Alexandrium minutum, which causes paralytic shellfish poisoning, is occurring with increasing frequency along European coasts. Current regulatory methods to analyze environmental samples are tedious and time consuming because they require taxonomists and involve animal experiments. New rapid detection methods, such as immunoassays, are needed to ensure a fast alert system and for field studies of algal ecodynamics. Rat monoclonal antibodies were raised and selected for their ability to specifically recognize a surface antigen for the A. minutum strain AM89BM from the Bay of Morlaix, France. A whole-cell ELISA was designed, leading to the selection of...
Tipo: Text Palavras-chave: Monoclonal antibody; Harmful algal bloom; Enzyme linked immunosorbent assay; Dinoflagellate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4647.pdf
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