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Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers Rev. Bras. Ciênc. Avic.
Gava,MS; Moraes,LB; Carvalho,D; Chitolina,GZ; Fallavena,LCB; Moraes,HLS; Herpich,J; Salle,CTP.
Brazilian poultry production is very efficient and demands maximum broiler performance. Therefore, digestive system pathologies have a relevant role. Considering it is difficult to obtain consistent information on intestinal morphometric analysis, this study aimed at establishing essential and clear criteria for the collection of intestinal segments for morphometric analysis. Fifteen 13-d-old broilers were sacrificed and three intestinal segments were collected per bird. Two 3-cm long sections were obtained from each of the intestinal segments. Samples were collected open or closed. The closed samples were transversely, hemicylindrically, or longitudinally sectioned. Samples were processed and stained with hematoxylin and eosin. The number of microscopic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Image; Intestine; Morphometry; Poultry; Villus.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200145
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