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| Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida. |
| Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014 |
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| Generoso,Andressa Leal; Viana,Alexandre Pio; Carvalho,Virginia Silva; Costa Júnior,Otalício Damásio da. |
| Abstract Dormancy in grape seeds slows the progress of many breeding programs of the crop, culminating in low uniformity and low germination percentages. Conventional methods used to overcome dormancy are time-consuming. Thus, in vitro seed germination emerges as a promising alternative to ensure the germination of grape seeds. In this study, we examined the in vitro germination and vigor of seedlings originating from seeds of ‘Red Globe’, ‘Italia’ and ‘Niagara Rosada’ grapes in growth media supplemented with five concentrations of gibberellic acid (GA3) (0, 1.41, 2.83, 4.24 and 5.66 µmol L-1) for 47 days. The use of GA3 increased seed germination percentage and seedling vigor, in the three varieties. Therefore, for the in vitro germination of seeds of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Gibberellic acid; Plant tissue culture; Grapes. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000502002 |
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