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| Genitsaris, Savvas; Monchy, Sebastien; Breton, Elsa; Lecuyer, Eric; Christaki, Urania. |
| The aim of this study was to analyze planktonic protistan assemblages under different environmental pressures at 2 adjacent coastal stations (inshore and offshore; similar to 6.4 km apart) in the eastern English Channel (EEC). Samples were collected between March 2012 and June 2013, and analyzed using bioinformatic analysis and tag pyrosequencing of the V2-V3 hypervariable region of the 18S rRNA gene. In addition to the taxonomic composition of the protistan communities, the detected operational taxonomic units (OTUs) were sorted into 6 major functional groups based on their trophic roles in marine systems. Comparisons of 13 environmental factors, including physical and chemical variables, indicated significant differences between the 2 stations. However,... |
| Tipo: Text |
Palavras-chave: Unicellular eukaryotes; Community organization; Environmental parameters; Biotic interactions; 18S rRNA; Pyrosequencing; Bioinformatics; Ecological tools. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00373/48450/48765.pdf |
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| Genitsaris, Savvas; Monchy, Sebastien; Viscogliosi, Eric; Sime-ngando, Telesphore; Ferreira, Stephanie; Christaki, Urania. |
| Previous microscopy-based studies in the eastern English Channel have revealed it to be a productive meso-eutrophic coastal ecosystem, characterized by strong repeating patterns in microplankton succession. The present study examines the seasonal structure of the entire protistan community from March 2011 to July 2013, using tag pyrosequencing of the V2-V3 hypervariable region of the 18S rRNA gene. A total of 1242 OTUs and 28 high-level taxonomic groups, which included previously undetected taxa in the area, were identified. The detected OTUs were considered according to taxon-specific traits, which included their trophic role, abundance and specialization level. Taxa differentiation based on specialization level rather than abundance was more informative... |
| Tipo: Text |
Palavras-chave: Unicellular eukaryotes; Tag pyrosequencing; 18S rRNA gene; Succession; Generalists; Specialists. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00373/48454/48738.pdf |
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| Christaki, Urania; Georges, Clement; Genitsaris, Savvas; Monchy, Sebastien. |
| The spatial and temporal community composition of microzooplankton (dinoflagellates and ciliates) was assessed in the Kerguelen area (Southern Ocean) during the KEOPS2 cruise in early spring (October-November) 2011. This naturally iron-fertilized region was characterized by a complex mesoscale circulation resulting in a patchy distribution of phytoplankton blooms. Collectively, 97 morphospecies of dinoflagellates and ciliates belonging to 41 genera were identified by microscopy, and 202 Alveolata-related OTUs (operational taxonomical units) were retrieved with tag-pyrosequencing. Microscopy and pyrosequencing data were in accordance, in that diatom-consuming dinoflagellates were the most enhanced taxa in the blooms. Dinoflagellates also showed significant... |
| Tipo: Text |
Palavras-chave: Dinoflagellates; Ciliates; Natural iron fertilization; Southern Ocean. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00351/46255/46080.pdf |
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