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	Provedor de dados Anais da ABC (AABC) (2) Autor Gil,Laura H.V.G. (2) Acioli-Santos,Bartolomeu (1) Bertani,Giovani R. (1) Calzavara-Silva,Carlos E. (1) Gomes,Ana L.V. (1) Maia,Rita C.C. (1) Marques Jr,Ernesto T.A. (1) Marques Jr.,Ernesto T.A. (1) Queiroz,Sabrina R.A. (1) Santos,Jefferson J.S. (1) Silva,Andréa N.M.R. (1) Mais... Palavra-chave Cloning technique (1) Dengue hemorrhagic fever (1) Homologous recombination (1) Molecular markers (1) Real-time PCR (1) Replicon (1) Reporter gene (1) Yellow fever virus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2009 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Early molecular markers predictive of dengue hemorrhagic fever Anais da ABC (AABC) Calzavara-Silva,Carlos E.; Gomes,Ana L.V.; Maia,Rita C.C.; Acioli-Santos,Bartolomeu; Gil,Laura H.V.G.; Marques Jr.,Ernesto T.A.. The management of acute dengue patients during outbreaks is a challenging problem. Most of the dengue fever cases are benign, but some cases develop into a severe and possibly lethal vasculopathy, known as dengue hemorrhagic fever. Early symptoms of dengue and hemorrhagic fever are very similar. An early differential diagnosis is needed to predict which of these two clinical presentations is crucial to proper patient care and public health management. This study evaluates the predictive potential of specific mRNA expression markers of dengue hemorrhagic fever using quantitative real-time PCR assays. Six candidate "dengue hemorrhagic fever specific signature genes" were evaluated and all showed good correlation among their transcription levels at early days... Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time PCR; Dengue hemorrhagic fever; Molecular markers. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000400006 Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique Anais da ABC (AABC) Queiroz,Sabrina R.A.; Silva,Andréa N.M.R.; Santos,Jefferson J.S.; Marques Jr,Ernesto T.A.; Bertani,Giovani R.; Gil,Laura H.V.G.. RNA replicon derived from Flavivirus genome is a valuable tool for studying viral replication independent of virion assembly and maturation, besides being a great potencial for heterologous gene expression. In this study we described the construction of subgenomic replicons of yellow fever virus by yeast-based homologous recombination technique. The plasmid containing the yellow fever 17D strain replicon (pBSC-repYFV-17D), previously characterized, was handled to heterologous expression of the green fluorescent protein (repYFV-17D-GFP) and firefly luciferase (repYFV-17D-Luc) reporter genes. Both replicons were constructed by homologous recombination between the linearized vector pBSC-repYFV-17D and the PCR product containing homologous 25 nucleotides ends... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cloning technique; Homologous recombination; Replicon; Reporter gene; Yellow fever virus. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100159 Registros recuperados: 2 Primeira ... 1 ... Última

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