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Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues BABT
Linan,Lamia Zuniga; Lima,Nadson Murilo Nascimento; Benatti,Carol; Xavier,Mariana; Rodrigues,Ana A.; Manenti,Flavio; Jardini,Andre; Maciel Filho,Rubens; Gilioli,Rovilson.
ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PMMA; Synthesis; Cytotoxicity; Viability; Mesenchymal cells..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100601
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Bartonellosis diagnosis requires careful evaluation BJID
Drummond,Marina Rovani; Gilioli,Rovilson; Velho,Paulo Eduardo Neves Ferreira.
Tipo: Info:eu-repo/semantics/article
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300002
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Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation BJM
Drummond,Marina Rovani; Pitassi,Luiza H. U.; Lania,Bruno G.; Santos,Silvio R. C. dos; Gilioli,Rovilson; Velho,Paulo E. N. F..
Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bartonella; Culture Media; False Positive Reactions; Polymerase Chain Reaction; Sheep-Blood Contamination.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200003
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