|
|
|
|
| |
|
|
| Lechable, Marion; Jan, Anne-cecile; Duchene, Axel; Uveira, Julie; Weissbourd, Brandon; Gissat, Loann; Collet, Sophie; Gilletta, Laurent; Chevalier, Sandra; Leclère, Lucas; Peron, Sophie; Barreau, Carine; Lasbleiz, Régis; Houliston, Evelyn; Momose, Tsuyoshi. |
| The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different... |
| Tipo: Text |
Palavras-chave: Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76820/77996.pdf |
| |
|
|
|
