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Registros recuperados: 10
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Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers Genet. Mol. Biol.
Palmieri,Darío A.; Bechara,Marcelo D.; Curi,Rogério A.; Monteiro,Jomar P.; Valente,Sérgio E.S.; Gimenes,Marcos A.; Lopes,Catalina R..
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis; Genetic diversity; Germplasm; Microsatellites; Molecular markers.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019
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Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence Genet. Mol. Biol.
Mota,Lígia S.L.S.; Curi,Rogério A.; Palmieri,Darío A.; Borges,Alexandre S.; Lopes,Catalina R.; Barbosa,José D.; Gimenes,Marcos A..
Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of the great adaptive capacity of these animals to tropical climates, together with the good productive and reproductive potential which make these animals an important animal protein source for poor and developing countries. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although the presence of non-silent and silent changes in the coding sequences and several alterations in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; GDF8; Myostatin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100016
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Identifying water stress-response mechanisms in citrus by in silico transcriptome analysis Genet. Mol. Biol.
Torres,Gisele A.M.; Gimenes,Marcos A.; Rosa Jr.,Vicente E. de; Quecini,Vera.
Water deficit is one of the most critical environmental stresses to which plants are submitted during their life cycle. The evolutionary and economic performance of the plant is affected directly by reducing its survival in the natural environment and its productivity in agriculture. Plants respond to water stress with biochemical and physiological modifications that may be involved in tolerance or adaptation mechanisms. A great number of genes have been identified as transcriptionally regulated for water deficit. EST sequencing projects provide a significant contribution to the discovery of expressed genes. The identification and determination of gene expression patterns is important not only to understand the molecular bases of plant responses but also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental stress; CitEST; Data mining; Tolerance mechanisms; Water deficit.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500018
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Effects of CSN3 and LGB gene polymorphisms on production traits in beef cattle Genet. Mol. Biol.
Curi,Rogério A.; Oliveira,Henrique N. de; Gimenes,Marcos A.; Silveira,Antonio C.; Lopes,Catalina R..
The objective of the present study was to estimate the allele and genotype frequencies of the CSN3/HinfI and LGB/HaeIII gene polymorphisms in beef cattle belonging to different genetic groups, and to determine the effects of these polymorphisms on growth and carcass traits in these animals, which are submitted to an intensive production model. Genotyping was performed on 79 Nelore, 30 Canchim (5/8 Charolais + 3/8 Zebu) and 275 crossbred cattle originating from the crosses of Simmental (n = 30) and Angus (n = 245) sires with Nelore females. Body weight, weight gain, dressing percentage, longissimus dorsi area and backfat thickness were fitted using the GLM procedure, and least square means of the genotypes were compared by the F test. The results showed...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Beef cattle; Polymorphisms; Candidate gene; Growth; Carcass.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200015
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Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers Genet. Mol. Biol.
Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016
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In silico evaluation of the Eucalyptus transcriptome Genet. Mol. Biol.
Vicentini,Renato; Sassaki,Flávio T.; Gimenes,Marcos A.; Maia,Ivan G.; Menossi,Marcelo.
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptome; Eucalyptus; Tissue-specific; Statistics; Differential expression.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400002
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Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks Genet. Mol. Biol.
Dantas,Alisson F.; Lopes,Renata M.; Fascineli,Maria L.; José,Solange C.B.R.; Pádua,Juliano G.; Gimenes,Marcos A.; Grisolia,Cesar K..
Abstract This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa L.; Phaseolus vulgaris L.; Methyl methanesulfonate; Cytogenetic test; Comet test.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100145
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Heterologous microsatellite primer pairs informative for the whole genus Arachis Genet. Mol. Biol.
Hoshino,Andrea Akemi; Bravo,Juliana Pereira; Angelici,Carla M.L.C.D.; Barbosa,Andréa V. Gobbi; Lopes,Catalina Romero; Gimenes,Marcos A..
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peanut; Genetic relationship; Molecular markers; Wild relatives.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016
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In silico analysis of phytohormone metabolism and communication pathways in citrus transcriptome Genet. Mol. Biol.
Quecini,Vera; Torres,Gisele A.M.; Rosa Jr,Vicente E. de; Gimenes,Marcos A.; Machado,Jeanne B. de M.; Figueira,Antonio V. de O.; Benedito,Vagner; Targon,Maria Luisa P.N.; Cristofani-Yaly,Mariângela.
Plant hormones play a crucial role in integrating endogenous and exogenous signals and in determining developmental responses to form the plant body throughout its life cycle. In citrus species, several economically important processes are controlled by phytohormones, including seed germination, secondary growth, fruit abscission and ripening. Integrative genomics is a powerful tool for linking newly researched organisms, such as tropical woody species, to functional studies already carried out on established model organisms. Based on gene orthology analyses and expression patterns, we searched the Citrus Genome Sequencing Consortium (CitEST) database for Expressed Sequence Tags (EST) consensus sequences sharing similarity to known components of hormone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Defense responses; Development; Plant growth regulators.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500002
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Genetic relationships among Arachis species based on AFLP Genet. Mol. Biol.
Gimenes,Marcos A.; Lopes,Catalina R.; Valls,Jose F.M..
Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI - ACC / Mse I - CTG, while the lowest was 108, with Eco RI - ACT / Mse I - CTT. In general, the genetic relationships established using AFLPs agreed with the classification established...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Arachis; Peanut; Taxonomic relationship.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300017
Registros recuperados: 10
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