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Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone 77
Ginani,Fernanda; Mota-Filho,Haroldo Gurgel; Vasconcelos Barreto,Mardem Portela e; Barboza,Carlos Augusto Galvão.
Bone remodeling is a process regulated by the interaction between cells and various molecules such as parathyroid hormone (PTH). The aim of the study was to evaluate the effect of different doses of PTH on osteoclast activity in a culture model of bone organs. Six-day-old male C57BL/6 mice (n=14) were euthanized and the calvariae were dissected and sectioned in the middle, keeping the periosteal and endosteal. The bone fragments were divided into three groups: Group I (control - without adding PTH), Group II (addition of 3 nM PTH) and Group III (30 nM PTH), all cultured in aMEM for up to 72 h osteoclast activity was evaluated by biochemical quantification of calcium released in the culture medium at intervals of 24, 48, and 72 h and by histomorphometric...
Tipo: Journal article Palavras-chave: Parathyroid hormone; Osteoclast; Bone remodeling; Organ culture techniques.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200033
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Use of Clinical Cases in a Virtual Learning Environment as an Approach to Teaching Human Embryology 77
Ginani,Fernanda; Gadelha Vasconcelos,Rodrigo; Galvão Barboza,Carlos Augusto.
The objective of this study was to evaluate the effectiveness of virtual learning as an approach to teaching human embryology. For this purpose, clinical cases comprising the six major topics of human embryology were organized into a blog in order to complement the content discussed in the classroom. Scientific articles were made available to reinforce theoretical contents and ethical-humanistic issues were addressed in discussion forums. The mean grades of the students at the end of the semester were compared with those of the previous semester when the method had not been applied. Student perceptions of blog effectiveness were evaluated using an electronic questionnaire. A substantial increase in mean grades was observed compared to the previous class....
Tipo: Journal article Palavras-chave: Teaching; Medical education; Embryology; Blog.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400022
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