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Stringari,Danyelle; Glienke,Chirlei; Christo,Daniel de; Maccheroni Jr.,Walter; Azevedo,João Lucio de. |
RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Guignardia; SCARS; Citrus Black Spot; Citrus; Diagnosis; RAPD. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500002 |
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Moreira,Mônica; Adamoski,Douglas; Sun,Jiufeng; Najafzadeh,Mohammad Javad; Nascimento,Mariana Machado Fidelis do; Gomes,Renata Rodrigues; Barbieri,Dicler de Sant'Anna; Glienke,Chirlei; Klisiowicz,Débora do Rocio; Vicente,Vânia Aparecida. |
The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Padlock probe; RCA; Streptococcus mutans. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054 |
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