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| Registros recuperados: 30 | |
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| Goarant, Cyrille; Merien, F. |
| Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular... |
| Tipo: Text |
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf |
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| Goarant, Cyrille; Regnier, France; Brizard, Raphael; Marteau, Anne-laure. |
| Vibriosis is a major disease problem in shrimp aquaculture, affecting all developmental stages, from larvae in hatchery tanks to juveniles and broodstock in growout ponds. However, bacterial strains responsible for vibriosis in the successive stages are usually considered to be different, and virulence specificity has been reported both at the species and at the stage levels. The so-called « Syndrome 93 » is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Penaeus stylirostris in growout ponds and broodstock tanks in New Caledonia. This pathology does not cause any mortality in hatchery or nursery phases. An experimental infection design using balneation of postlarvae and early juveniles in V. penaeicida suspensions was used to... |
| Tipo: Text |
Palavras-chave: Penaeus stylirostris; Vibrio sp.; Diseases and their control crustaceans. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/1998/publication-383.pdf |
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| Goarant, Cyrille. |
| Dans le cadre du suivi épidémiologique de Vibrio penaeicida dans les élevages de crevettes du Territoire, il importait de considérer l'éventualité d'une transmission pseudo-verticale du pathogène, c'est-à-dire des géniteurs aux larves et post-larves. Ce suivi a concerné des productions de quatre écloseries du Territoire (Ecloserie du Nord, Ecloserie de Mara, Ecloserie de Montagnès et Ecloserie de la SASV) à différentes saisons, et sur différents stades de développement. Le portage de V. penaeicida est confirmé sur les géniteurs, mais la transmission pseudo-verticale semble exclue au vu des résultats de ce suivi. |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00274/38542/37062.pdf |
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| Goarant, Cyrille; Merien, Fabrice; Berthe, Franck; Mermoud, Isabelle; Perolat, Philippe. |
| A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR... |
| Tipo: Text |
Palavras-chave: Vibrio spp.; Shrimp; Molecular typing. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-2734.pdf |
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| Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques. |
| Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two... |
| Tipo: Text |
Palavras-chave: Vibriosis; Survival; Growth; Penaeus Litopenaeus stylirostris; Shrimp; Cross breeding; Genetic improvement. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4316.pdf |
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| Le Roux, Frederique; Labreuche, Yannick; Davis, Brigid M.; Iqbal, Naeem; Mangenot, Sophie; Goarant, Cyrille; Mazel, Didier; Waldor, Matthew K.. |
| Vibrioses are the predominant bacterial infections in marine shrimp farms. Vibrio nigripulchritudo is an emerging pathogen of the cultured shrimp Litopenaeus stylirostris in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have revealed that recent pathogenic V. nigripulchritudo isolates from New Caledonia all cluster into a monophyletic clade and contain a small plasmid, pB1067. Here, we report that a large plasmid, pA1066 (247 kb), can also serve as a marker for virulent V. nigripulchritudo, and that an ancestral version of this plasmid was likely acquired prior to other virulence-linked markers. Additionally, we demonstrate that... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14196/11469.pdf |
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| Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B. |
| Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida... |
| Tipo: Text |
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf |
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| Goarant, Cyrille. |
| Une étude de typage moléculaire par AP-PCR de souches de Vibrio impliqués dans des épisodes de mortalité d'élevages de crevettes en Nouvelle-Calédonie (Syndrome 93) et au Japon a été réalisée fin 1996 avec le concours de l' Institut Pasteur de Nouvelle-Calédonie. Elle nous a permis une identification rapide des isolats en terme de génospecies ainsi que des discriminations d' intérêt épidémiologique entre les isolats du génospecies majoritaire. Ainsi, des sous-groupes ont été identifiés au sein de l' espéce majoritaire : V. penaeicida. Certains de ces sous-groupes correspondent à des topotypes et permettent de discriminer les isolats du Japon de ceux de Nouvelle-Calédonie, ainsi que ceux de deux baies différentes de Nouvelle Calédonie, distinctes de 50 km... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00274/38541/37061.pdf |
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| Le Moullac, Gilles; Boglio, Eric; Goarant, Cyrille. |
| Une substance immunostimulante administrée par voie orale est testée chez la crevette Penaeus stylirostris. Cette expérimentation est réalisée à la station de St Vincent afin de se rapprocher des conditions du Syndrôme 93. Les effets sont mesurés pendant 2 semaines sur des paramètres hématologiques (hémocytes totaux, cellules à grands granules, coloration spécifique : cellules incorporant le rouge neutre). D'autre part un stress des crevettes en cage sur un fond réduit en bassin constitue le challenge. L'administration de l'immunostimulant par voie orale augmente significativement le nombre des hémocytes totaux, ainsi que les cellules incorporant le rouge neutre. Cependant le stress de la mise en cage sur fond réduit n'a pas déclenché de mortalité de type... |
| Tipo: Text |
Palavras-chave: Penaeus stylirostris; Syndrome 93; Immunostimulant; Hémocyte; Stade de mue.. |
| Ano: 1997 |
URL: https://archimer.ifremer.fr/doc/00680/79245/81762.pdf |
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| Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene. |
| Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical... |
| Tipo: Text |
Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| Lemonnier, Hugues; Bernard, E; Boglio, E; Goarant, Cyrille; Cochard, Jean-claude. |
| Penaeid shrimp reared in earthen ponds are exposed to sediment, which can, in some instances, induce a stress. In seawater, the osmoregulatory capacity (hyporegulation) is a useful tool to compare the physiological condition of shrimp exposed to various stressors. By keeping some shrimp in cages at different locations of a single pond heterogeneous in terms of sediment quality, it was possible, using osmotic pressure (OP), to identify some locations where the stress was maximum and some others where it was minimum. Simultaneously, sediment samples were taken and analysed in order to evaluate some physico-chemical parameters that could be related to the stress observed in the shrimps kept in the cages. This approach allowed to show a significant positive... |
| Tipo: Text |
Palavras-chave: PH; Sediments; Stress; Osmotic pressure; Shrimp physiology. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-1866.pdf |
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| De Lorgeril, Julien; Gueguen, Yannick; Goarant, Cyrille; Goyard, Emmanuel; Mugnier, Chantal; Fievet, Julie; Piquemal, D; Bachere, Evelyne. |
| Understanding of antimicrobial defence mechanisms of penaeid shrimp should help in the design of efficient strategies for the management and disease control in aquaculture. In this study, we have specifically analysed the expression in circulating hemocytes of antimicrobial peptides (AMPs) encoding genes, such as PEN2 and PEN3, ALF, crustin, lysozyme and a putative cysteine-rich peptide. We evidenced a relationship between the level of expression of some AMPs and the successful response of the shrimp, Litopenaeus stylirostris, to circumvent a pathogenic Vibrio penaeicida infection. Additionally, significant differences in some AMP transcript amounts are evidenced between control, non-selected shrimp line and the third generation breeding of shrimp selected... |
| Tipo: Text |
Palavras-chave: Real time PCR; Cysteine rich peptide; Crustin; Anti LPS factor; Lysozyme; Penaeidins; Immune response; Vibrio penaeicida; Penaeid; Decapoda; Crustacean. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4524.pdf |
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| Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly. |
| New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government... |
| Tipo: Text |
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf |
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| Saulnier, Denis; Reynaud, Yann; Arzul, Isabelle; Miossec, Laurence; Le Roux, Frédérique; Goarant, Cyrille. |
| According to the world organisation for animal health (OIE) an emerging disease is defined as a recently admitted serious illness, whose aetiology can, or not, have already been established, and which is likely to be propagated within a population or between populations, for example at the time of international exchanges of aquatic animals and/or products of aquatic animals. Even though the emerging diseases that affect human health have been much studied, those which affect marine organisms and species of aquaculture interest in particular are poorly documented. By restricting emergence to only infectious diseases, we aim at presenting in a non-exhaustive way some scenarios of the emergence of the diseases of aquacultured species by illustrating them with... |
| Tipo: Text |
Palavras-chave: Cyprinus carpio; Ostrea edulis; Bonamia ostreae; Peneides; Vibrio nigripulchritudo; Herpesvirus; Agent pathogène. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2980.pdf |
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| Mugnier, Chantal; Zipper, Etienne; Goarant, Cyrille; Lemonnier, Hugues. |
| The effect of ambient ammonia, hypoxia and combination of both on survival and the physiological and immunological response of the blue shrimp Litopenaeus stylirostris in relation to molt stage was studied. Shrimp were submitted to 44.0-71.5 mg 1(-1) total ammonia-N corresponding to 2.0 mg 1(-1) unionized ammonia NH3-N and/or to 1.5 mg O-2 1(-1) (4.3 kPa) for 24 hours. Survival was recorded and the molt stages of both dead and surviving shrimp determined. Only shrimp in intermolt and premolt stages were sampled for analysis of haemolymph. Haemolymph was assayed for osmoregulatory capacity (OC), magnesium ion (Mg2+), calcium ion (Ca2+), total proteins, oxyhaemocyanin, lactate, glucose and total haemocyte count (THC). Low mortalities were recorded for... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4092.pdf |
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| Le Moullac, Gilles; Phillip De Laborie, Loic; Saulnier, Denis; Goarant, Cyrille; Dehasque, Marleen. |
| Anderson (1992) y Raa (1996) han escrito impresionantes críticas acerca del uso de inmunoestimulantes en acuacultura. La mayoría de los trabajos citados se refieren principalmente al cultivo de peces y pocos acerca del camarón. Así que este trabajo está enfocado al camarón y las pocas pruebas publicadas. Varios de los inmunoestimulantes y su manera de actuar sobre el sistema inmune en camarones se describen brevemente, así como los métodos usados para administrar las sustancias inmunoestimulatorias para camarón. La dificultad para experimentar en este campo está señalada con algunos ejemplos. |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00029/14011/11197.pdf |
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| Registros recuperados: 30 | |
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