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| Abba,M.C.; Gómez,M.A.; Golijow,C.D.. |
| In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a small native polyacrylamide gel, followed by a rapid silver staining procedure. In order to validate the technique and to obtain the allele patterns for the DQA1 gene, 50 cervical samples were typed using this methodology and the commercial Amplitype® HLA DQA1 Amplification and Typing kit. All the alleles detected with the kit were characterized by the LIS-SSCP approach.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HLA-DQA1; PCR; LIS-SSCP. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700005 |
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| Golijow,C.D.; Giovambattista,G.; Rípoli,M.V.; Dulout,F.N.; Lojo,M.M.. |
| Many cattle breeds have been subjected to high selection pressure for production traits. Consequently, population genetic structure and allelic distribution could differ in breeds under high selection pressure compared to unselected breeds. Analysis of <FONT FACE="Symbol">k</font>-casein, <FONT FACE="Symbol">a</font>S1-casein and prolactin gene frequencies was made for Argentine Creole (AC) and Argentine Holstein (AH) cattle herds. The calculated FST values measured the degree of genetic differentiation of subpopulations, depending on the variances of gene frequencies.The AC breed had considerably more variation among herds at the <FONT FACE="Symbol">a</font>S1-casein and <FONT FACE="Symbol">k</font>-casein... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300018 |
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