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	Provedor de dados Biological Sciences (1) Autor Franceschi, Cristina do Rosário Batista (1) Gomes, Lucas Roberto Pereira (1) Ribas, Luciana Lopes Fortes (1) Palavra-chave Biotecnologia (1) Botânica epiphytic orchid (1) In vitro propagation (1) Protocorm-like body 2.03.00.00-0 (1) Protocorms (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2015 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture Biological Sciences Gomes, Lucas Roberto Pereira; Franceschi, Cristina do Rosário Batista; Ribas, Luciana Lopes Fortes. An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotecnologia; Botânica epiphytic orchid; In vitro propagation; Protocorms; Protocorm-like body 2.03.00.00-0. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276 Registros recuperados: 1 Primeira ... 1 ... Última

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