| ABSTRACT The addition of levels of ethanol extract of brown propolis was evaluated by assessing diet degradation in rumen fluid and predicting cumulative in vitro gas production by nonlinear (dual pool logistic and exponential) models. A total of 35 g of crude propolis were extracted in 65 mL of cereal alcohol (95% ethanol). In a completely randomized factorial design, the experimental diets combined four concentrations of extracted propolis diluted in cereal alcohol (0, 50, 70, and 100% of propolis extract) and supplementation doses (4, 8, 12, 16, and 20 mL/kg dry matter), tested in triplicate. Diet (400 g/kg Tifton hay and 600 g/kg concentrate) was incubated for 96 h carried out three times in three different weeks. There was significant interaction... |
