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Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco. |
Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005 |
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Rodrigues,Cristina Crespo; Höfling,José Francisco; Boriollo,Marcelo Fabiano Gomes; Rodrigues,Janaina Aparecida de Oliveira; Azevedo,Ricardo Antunes de; Gonçalves,Reginaldo Bruno; Gomes,Luiz Humberto; Tavares,Flávio César Almeida. |
The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Numerical analyzes; SDS-PAGE; C. albicans; Human anatomical sites. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100006 |
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Kamiya,Regianne Umeko; Taiete,Tiago; Gonçalves,Reginaldo Bruno. |
The colonization and accumulation of Streptococcus mutans are influenced by various factors in the oral cavity, such as nutrition and hygiene conditions of the host, salivary components, cleaning power and salivary flow and characteristics related with microbial virulence factors. Among these virulence factors, the ability to synthesize glucan of adhesion, glucan-binding proteins, lactic acid and bacteriocins could modify the infection process and pathogenesis of this species in the dental biofilm. This review will describe the role of mutacins in transmission, colonization, and/or establishment of S. mutans, the major etiological agent of human dental caries. In addition, we will describe the method for detecting the production of these inhibitory... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus mutans; Mutacin; Mutacintyping. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400001 |
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Rosa,Rosimeire Takaki; Napimoga,Marcelo Henrique; Höfling,José Francisco; Gonçalves,Reginaldo Bruno; Rosa,Edvaldo Antonio Ribeiro. |
Twenty-one Streptococcus mutans strains were clustered by Multilocus Enzyme Electrophoresis (MLEE). Six isoenzymes showed strong infra-specific discriminatory power (M1P, MPI, PLP, NSP, GOT, and LAP). MLEE is a robust technique that may be used to explore clonal diversity of S. mutans isolates in epidemiological surveys. |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Streptococcus mutans; Isoenzymes; Clonal variability. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100003 |
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