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Ribas,Rosineide Marques; Gontijo-Filho,Paulo Pinto; Darini,Ana Lúcia da Costa. |
In this study, for detection of methicillin-resistant S. aureus (MRSA), a mecA multiplex PCR-based amplification was compared with the 1 µg oxacillin disk diffusion test, detection of minimal inhibitory concentration (MIC), and screening in agar with 4% NaCl and 6 µg/mL oxacillin. Among 24 isolates obtained from blood, mecA gene was detected in only 16 (66.7%) isolates by multiplex PCR. The MIC test showed a range of resistance to oxacillin from 0.19 to 512 µg/mL, among these isolates. Data obtained by screening and dilution tests showed that sensitivity to methicillin was 80.0% and 72.8%, respectively, when compared with the presence of mecA gene (multiplex). All isolates, including the negatives, when revaluated for mecA gene by PCR were positive.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multiplex PCR; MRSA; Beta-lactamase; MecA gene. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500012 |
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Brito,Denise Von Dolinger de; Oliveira,Elias Jose; Darini,Ana Lúcia da Costa; Abdallah,Vânia Olivetti Sttephen; Gontijo-Filho,Paulo Pinto. |
The study documents the occurrence of two subsequent outbreaks in the NICU of HC-UFU, caused by epidemic strains of multiresistant Pseudomonas aeruginosa and Acinetobacter baumannii, that occurred between March/01 and September and between October/01 and March/02, respectively. The P. aeruginosa outbreak included seven neonates with conjunctivitis and three with bacteremia. A case-control study was conducted for the A. baumannii outbreak, with 11 and 22 neonates, respectively. The isolates of A. baumannii were resistant to gentamacin and ciprofloxacin. P. aeruginosa isolates were resistant to ampicillin/sulbactam gentamicin and ciprofloxacin. The hands of healthcare workers and environmental cultures were negative. The outbreak of P. aeruginosa resulted in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Outbreak; Neonate; Acinetobacter baumannii; Pseudomonas aeruginosa. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500009 |
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