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Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line Electron. J. Biotechnol.
Crispo,Martina; Schlapp,Geraldine; Cárdenas-Rodríguez,Magdalena; González-Maciel,Dolores; Rumbo,Martín.
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize aspects of the procedure. Three different concentrations (0.5, 1.0 and 4.0 ng/µl) of the DNA construction to microinject a total of 1981 zygotes has been tested. Intact/injected embryos, pregnancy and birth rate, survival of pups 7 days after birth, number of transgenic pups and overall transgenic efficiency was registered and analyzed by Z test of proportions for each group. Results: A total of seven transgenic founders were detected for the three DNA...
Tipo: Journal article Palavras-chave: DNA concentration; Microinjection; Reporter systems.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600014
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