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Hirayama, Kentaro; Taufik, Epi; Kikuchi, Megumi; Nakamura, Tadashi; Fukuda, Kenji; Saito, Tadao; Newgrain, Keith; Green, Brian; Messer, Michael; Urashima, Tadasu. |
Previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. To clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. Neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two samples of milk of the common wombat and characterized by H-1-nuclear magnetic resonance spectroscopy. The structures of six neutral saccharides were found to be Gal(1-4)Glc (lactose), Gal(1-3)Gal(1-4)Glc (3'-galactosyllactose), Gal(1-3)Gal(1-3)Gal(1-4)Glc... |
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Palavras-chave: Common wombat; Marsupial; Milk oligosaccharide; Vombatus ursinus. |
Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4471 |
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Urashima, Tadasu; Yamamoto, Tomoko; Hirayama, Kentaro; Fukuda, Kenji; Nakamura, Tadashi; Saito, Tadao; Newgrain, Keith; Merchant, Jim; Green, Brian; Messer, Michael. |
Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by H-1 - nuclear magnetic resonance spectroscopy and matrix - assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3) Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4) Glc, Gal(beta 1-3) Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-3) Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4)... |
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Palavras-chave: Tiger quoll; Milk oligosaccharides; Marsupials. |
Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4528 |
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