|
|
|
| Registros recuperados: 32 | |
|
|
| Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete. |
| ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673 |
| |
|
| |
|
|
| Mariano,André Bellin; Kovalhuk,Leonardo; Valente,Caroline; Maurer-Menestrina,Juliana; Pereira-Netto,Adaucto Bellarmino; Guerra,Miguel Pedro; Carnieri,Eva Gunilla Skare. |
| A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria angustifolia; Callus; Plant mitochondria; Respiratory chain. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006 |
| |
|
|
| Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro. |
| Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp.. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 |
| |
|
| |
|
|
| Booz,Maristela Raitz; Kerbauy,Gilberto B.; Guerra,Miguel Pedro; Pescador,Rosete. |
| The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid; Pineapple guava; Plant growth. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000400003 |
| |
|
|
| Oltramari,Ana Carla; Dal Vesco,Lirio Luiz; Pedrotti,Enio Luiz; Ducroquet,Jean-Pierre Henri Joseph; Nodari,Rubens Onofre; Guerra,Miguel Pedro. |
| Visando ao desenvolvimento de um novo protocolo para a micropropagação da goiabeira serrana (Acca sellowiana (Berg) Burret) foram estabelecidos experimentos com segmentos nodais e microestacas. As citocininas 6-Benzilaminopurina (BAP), Cinetina (Kin) e 2-Isopenteniladenina (2-iP) foram adicionadas ao meio de cultura Woody Plant Midium-WPM, visando à proliferação de brotações múltiplas dos genótipos 53B-7, 101, 529 e 152-12 x 458. As microestacas obtidas in vitro foram submetidas a diferentes períodos de indução, em 20 miM de ácido indolbutírico (AIB) e, posteriormente, transferidas para meio de cultura isento desse fitorregulador para a indução de raízes ou, alternativamente, submetidas a diferentes concentrações e tempos de exposição em AIB e transferidas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultura de tecidos; Organogênese; Taxa de regeneração; Enraizamento in vitro e ex vitro. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100010 |
| |
|
| |
|
| |
|
| |
|
|
| Auler,Neiva Maria Frizon; Reis,Maurício Sedrez dos; Guerra,Miguel Pedro; Nodari,Rubens Onofre. |
| The objective of this study was to generate information relative to the allele distribution and dynamics within and among populations of Araucaria angustifolia, a naturally-occurring conifer in the south of Brazil, being known popularly as "pinheiro-do-Paraná", "araucaria" or pine tree. In order to elucidate the levels and the distribution of the genetic variability, the population's genetic structure and the genetic distance among natural populations of this species with different levels of disturbance in different geographical areas were studied in detail. For this, samples of leaf tissue were collected from 328 adult individuals in nine natural populations in Santa Catarina State. To analyze the samples, the allozyme technique was applied in starch gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pinheiro; Forest degradation; Genetic diversity; Genetic erosion. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300014 |
| |
|
|
| Schlögl,Paulo Sérgio; Santos,André Luis Wendt dos; Vieira,Leila do Nascimento; Floh,Eny Iochevet Segal; Guerra,Miguel Pedro. |
| Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seed development; Brazilian Pine; Embryogenesis-regulating genes; Zygotic embryogenesis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025 |
| |
|
|
| Santos,Karine Louise dos; Welter,Leocir José; Dantas,Adriana Cibele de Mesquita; Guerra,Miguel Pedro; Ducroquet,Jean Pierre Henri Joseph; Nodari,Rubens Onofre. |
| The pineapple guava (Acca sellowiana), known in portuguese as the goiabeira-serrana or "Feijoa", is a native fruit tree from southern Brazil and northern Uruguay that has commercial potential due to the quality and unique flavor of its fruits. Knowledge of genetic variability is an important tool in various steps of a breeding program, which can be facilitated by the use of molecular markers. The conservation of repeated sequences among related species permits the transferability of microsatellite markers from Eucalyptus spp. to A. sellowiana for testing. We used primers developed for Eucalyptus to characterize A. sellowiana accessions. Out of 404 primers tested, 180 amplified visible products and 38 were polymorphic. A total of 48 alleles were detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Feijoa sellowiana; Genetic diversity; Goiabeira-serrana; Pineapple-guava; Transferability. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100014 |
| |
|
|
| Magalhães,Janaina S; Santos,Maria do Desterro M dos; Cunha Filho,Francisco N da; Blumer,Lucimara; Guerra,Miguel Pedro; Torres,Antônio Carlos. |
| A diferenciação e o desenvolvimento de embriões somáticos foram observados em 15 genótipos de batata-doce a partir de explantes de ápices caulinares com um ou dois primórdios foliares. A embriogênese foi induzida utilizando o meio composto de sais minerais MS e vitaminas, 3% de sacarose, 0,2% de phytagel, suplementado com 2,4-D (0,0; 0,5; 1,0 e 2,0 mg/L). As culturas foram mantidas no escuro à temperatura de 27ºC. A adição de 2,4-D foi necessária para a indução de calo embriogênico nos genótipos '92', '94', '184', '188', '319', '370', '374', '442', '449', '5491, '594', '645', 'PI3138463', 'White Star' e 'Jewel'. Os clones '169', '202', '605', '633' e '643' não responderam à indução de calo embriogênico. Os calos embriogênicos apresentaram a coloração... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipomoea batatas; Embriogênese adventícia; Micropropagação; Cultura de tecidos. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000100016 |
| |
|
|
| Guerra,Miguel Pedro; Dal Vesco,Lírio Luiz; Pescador,Rosete; Schuelter,Adilson Ricken; Nodari,Rubens Onofre. |
| No Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal do CCA-UFSC, nos anos de 1993 a 1996, foram testadas 24 combinações de tratamentos envolvendo dois genótipos de abacaxizeiro (Ananas comosus (L.) Merr.), seis combinações dos fitorreguladores ácido naftalenoacético (ANA) e 6-benzilaminopurina (BAP), e os meios de cultura líquidos e geleificados, com o objetivo de estabelecer um protocolo regenerativo para a micropropagação do abacaxizeiro. A taxa de regeneração comportou-se de forma quadrática para a maioria das combinações testadas. O meio de cultura MS (Murashige & Skoog, 1962), líquido, adicionado de ANA (2,7 µM) e BAP (4,4 µM), proporcionou uma taxa média de regeneração de 19,7 brotos/explante. A magnitude do efeito genotípico... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultura de tecidos; Taxa de regeneração; Ananas comosus; Propagação clonal. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000900005 |
| |
|
|
| Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da. |
| The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008 |
| |
|
|
| Degenhardt,Juliana; Ducroquet,Jean Pierre; Reis,Mauricio Sedrez dos; Guerra,Miguel Pedro; Nodari,Rubens Onofre. |
| A variância ambiental costuma ser bastante significativa em características de produção e de frutos, a maioria de herança quantitativa. Este estudo teve por objetivo avaliar o efeito de anos e determinar o número de anos necessários para avaliação precisa do peso do fruto, peso de casca, comprimento, diâmetro, relação comprimento/diâmetro, rendimento de polpa e sólidos solúveis totais dos frutos de goiabeira-serrana (Acca sellowiana), em três anos de avaliação. Com exceção de sólidos solúveis totais, o efeito de anos representou grande parte da variância total. A partir do coeficiente de repetibilidade, determinou-se que seriam necessários no mínimo de quatro a seis anos de avaliação para seleção das plantas, com precisão de 80%. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acca sellowiana; Herdabilidade; Fator ambiental; Variação genética. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000900012 |
| |
|
| |
|
|
| Dantas,Adriana Cibele de Mesquita; Boneti,José Itamar; Nodari,Rubens Onofre; Guerra,Miguel Pedro. |
| The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malus; Marubakaido; M9; Culture medium; Carbon source. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600011 |
| |
|
|
| Schuck,Mariane Ruzza; Moreira,Flavia Maia; Voltolini,José Afonso; Guerra,Miguel Pedro; Grando,Maria Stella; Silva,Aparecido Lima da. |
| A variedade Goethe é símbolo da vitivinicultura da região de Urussanga, sul do Estado de Santa Catarina, a qual, atualmente, busca a Indicação Geográfica da Uva e do Vinho Goethe. Para isto, um dos requisitos necessários é a identificação precisa do material genético. Os marcadores microssatélites constituem a ferramenta molecular mais utilizada para a identificação varietal de videira em todo o mundo e têm a capacidade de produzir um perfil genético único para cada material vitícola. O objetivo deste trabalho foi caracterizar duas seleções de uva 'Goethe', presentes no município de Urussanga, por meio de marcadores moleculares microssatélites, visando a atender aos requisitos de denominação de origem e indicação de procedência controlada. A extração do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; Identificação varietal; Marcadores microssatélites; Variabilidade intravarietal. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000300023 |
| |
| Registros recuperados: 32 | |
|
|
|
