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Bioactivities of latexes from selected tropical plants Plantas Medicinales
Guerrero,Ricardo O; Guzmán,Ángel L.
Fifteen latexes from selected tropical plants were collected in Puerto Rico, Guadalupe and Ecuador and evaluated by 2 bioassays. The tests carried out were the brine shrimp lethality test (BSLT) and the DNA-methyl green (DNA-MG) interaction. An additional assay, antibacterial activity, was performed on the bark/latex extract of Mammea americana L. The results indicated that some of these latexes are bioactive. On the BSLT assay, the latexes of Euphorbia neriifolia L. and Sapium laurocerasus Desf. displayed LC50 values of 76,7 and 7,1 µg/mL, respectively. Moreover, the dichloromethane (DCM) fraction of the bark/latex of M. americana L., presented a LC50 1,1 mg/mL. In addition, in the DNA-MG interaction, this particular fraction exhibited an IC50 211,8...
Tipo: Journal article Palavras-chave: PLANTS; MEDICINAL PLANT EXTRACTS DNA METHYLGREEN PUERTO RICO GUADALUPE ECUADOR BIOLOGICAL ASSAY.
Ano: 2004 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962004000100015
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Inhibition of aldose reductase by herbs extracts and natural substances and their role in prevention of cataracts Plantas Medicinales
Guzmán,Ángel; Guerrero,Ricardo O.
Cataractogenesis is a common complication that occurs in diabetes mellitus. Aldose reductase is a lens enzyme probably involved in the development of this eye problem. The purpose of this investigation was to screen plant extracts for aldose reductase inhibitors (ARI) and to investigate their possible influence in diabetic cataractogenesis prevention. 13 plants and 3 natural products were randomly selected for our experiment. The 19 extracts originated from plant material which was extracted with ethanol, water and DCM, and assessed for inhibitors of aldose reductase. This enzyme was isolated from bovine lenses homogenates. The enzyme was incubated in a reaction mixture containing 50 mM Na-phosphate buffer (pH 6.2), NADPH, 400 mM LiSO4 and...
Tipo: Journal article Palavras-chave: Puerto Rico; Plant extracts; Aldose reductase inhibition; Cataractogenesis prevention; Prevención de cataratogénesis.
Ano: 2005 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962005000300005
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Specific bioassays with selected plants of Bangladesh Plantas Medicinales
Guerrero,Ricardo O; Mahmud,T; Khan,H; Casañas,Bárbara; Morales,Mara.
Summary Bangladesh is an Asian country where only 20 per cent of the people are provided with modern healthcare services while the rest 80 per cent are dependent on traditional plant-based systems. Moreover, it is estimated that only 500 medicinal plant species had been recorded in Bangladesh out of 1,900 species regarded as having medicinal value. Purpose: Sixteen collections of medicinal plants of different families were extracted with several solvents (Ethanol 95%, water, chloroform, ethyl ether). The resulting extracts were subjected to five different specific bioassays: 1. Brine shrimp lethality test; 2. Antioxidant activity; 3. Inhibition of xanthine oxidase (XO); 4. Inhibition of ß-glucosidase, and 5. Inhibition of acetylcholinesterase. Methods....
Tipo: Journal article Palavras-chave: Bangladesh; Plant extracts; Brine shrimp lethality test; Antioxidant activity; Xanthine oxidase inhibition; SS-glucosidase inhibition; Acetylcholinesterase inhibition.
Ano: 2004 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962004000200009
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