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Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Lu,S-B; Huo,Y-L; Guo,B; Guan,Z-P; Wei,Y-H.
Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until 42 days from culturing and then decreased sharply. Malondialdehyde...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Physiological; Biochemical; Organogenesis; Somatic embryogenesis; HBsAg-transgenic; Cherry tomato; Mutant.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200013
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Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008
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