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| Wu,Yuni; Xu,Youhua; Shen,Yali; Wang,Cuicui; Guo,Gaili; Hu,Tiantian. |
| The objective of this study was to evaluate the effects of tetramethylpyrazine (TMP) in combination with arsenic trioxide (As2O3) on the proliferation and differentiation of HL-60 cells. The HL-60 cells were treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively. The proliferative inhibition rates were determined with MTT. Differentiation was detected by the nitroblue tetrazolium (NBT) reduction test, Wright’s staining and the distribution of CD11b and CD14. Flow cytometry was used to analyze cell cycle distribution. RT-PCR and Western blot assays were employed to detect the expressions of c-myc, p27, CDK2, and cyclin E1. Combination treatment had synergistic effects on the proliferative inhibition rates. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetramethylpyrazine; Acute promyelocytic leukemia; Arsenic trioxide; HL-60 cell; Proliferation; Differentiation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300002 |
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