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Yu,Yi; Chang,De; Xu,Huiwen; Zhang,Xuelin; Pan,Lei; Xu,Chou; Huang,Bing; Zhou,Hong; Li,Jia; Guo,Jun; Liu,Changting. |
Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study was to characterize the effects of the pneumococcal dprA gene on the pathogenesis of S. pneumoniae. To construct a dprA-deficient pneumococcal strain, the dprA gene of the S. pneumoniae strain D39 was inactivated. The virulence of this dprA-deficient strain, designated ΔD39, was compared with that of the wild-type strain by evaluating their respective capabilities to adhere to human pulmonary epithelial cells (PEC-A549) and by analyzing their choline-binding... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; DprA; Transformation; Virulence. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200225 |
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Chen,Lanlan; Zhu,Zaibiao; Guo,Qiaosheng; Guo,Jun; Huang,Zhigang; Shi,Yongtao; Wen,Zhencui. |
ABSTRACT: Monochasma savatieri Franch. ex Maxim is a perennial, parasitic herb used in traditional Chinese medicine and its wild resources have decreased sharply in recent years due to destructively harvesting and habitat destruction. Haustorium formation is a key event of parasites, but the concentrations of haustorium-inducing factors vary with species and cultivation conditions. In this study, we investigated the effects of the 2,6-dimethoxy-p-benzoquinone (DMBQ) concentration and cultivation density on the growth traits, haustorium formation and biomass of M. savatieri in the absence of a host plant. The results showed that both the DMBQ concentration and cultivation density regulated growth traits, haustorium formation and biomass in M. savatieri. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial cultivation; Density; DMBQ; Fuzzy membership function; Growth promotion. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300202 |
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