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| Tian,Hongtao; Tan,Jianxin; Zhang,Lifang; Gu,Xinxi; Xu,Wentao; Guo,Xinghua; Luo,Yunbo. |
| The effects of the expression of a small heat shock protein (shsp) gene from Streptococcus thermophilus on stress resistance in Lactococcus lactis under different environmental stresses were investigated in this study. pMG36e-shsp, an expression vector, was first constructed by inserting a shsp open reading frame (ORF) cloned from S. thermophilus strain St-QC into pMG36e. Then, a food-grade expression vector, pMG-shsp, was generated by deleting the erythromycin resistance gene from pMG36e-shsp. The transformation rate of pMG-shsp was comparable to that of pMG36e-shsp when each of these two vectors was introduced into L. lactis. These results demonstrated that the shsp ORF could successfully used as a food-grade selection marker in both pMG-shsp and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shsp gene; Streptococcus thermophilus; Lactococcus lactis; Food-grade vector; Stress resistance. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300043 |
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