| ABSTRACT: Soil microorganisms are influenced by climate change. However, the effect of climate change on soil cultivable bacteria are unclear. In this study, the composition and diversity of the soil cultivable bacterial community were explored by a dilution–plate method, PCR, and 16S rRNA sequencing in a desert steppe of northern China after repeated NH4NO3 amendments and precipitation manipulation for seven years. The experimental treatments were as follows: control (CK), N addition (+N), N addition plus water addition (+N+W), and N addition plus water reduction (+N-W). Among the treatment groups, 11 genera and 17 bacterial species were isolated. Nitrogen addition and precipitation manipulation significantly increased the number of cultivable bacteria in... |
