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| Tsay,Hsin-Sheng; Ho,Hui-Min; Gupta,Sushim K; Wang,Chang-Sheng; Chen,Pei-Tsu; Chen,Emily Chin-Fun. |
| In the present study, a novel plant transformation system for Doritaenopsis and Phalaenopsis has been developed. The pollen-mediated activation tagging system was established by artificial pollination. The pollens, co-cultured with Agrobacterium tumefaciens strain EHA105 harbouring an activation tagging vector (pTAG-8), were used for pollination. In order to optimize the transformation efficiency, several factors (concentration of A. tumefaciens, concentration of acetosyringone during co-cultivation and the duration of co-cultivation) known to influence Agrobacterium-mediated DNA transfer were examined. A concentration of 0.5-1 x 10(8) CFU/ml for A. tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were found to be the optimal condition... |
| Tipo: Journal article |
Palavras-chave: Activation tagging; Agrobacterium tumefaciens; Doritaenopsis cultivars; Phalaenopsis amabilis; Transformation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400009 |
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