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| Coello,Christian Yanelly; Miceli,Clara Luz; Orantes,Carolina; DenDooven,Luc; Gutiérrez,Federico Antonio. |
| An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation. |
| Tipo: Journal article |
Palavras-chave: 6-benzyladenine; Indole-3-acetic acid; A-naftalenacetic acid; Gibberellic acid; Shoot induction; Rooting. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003 |
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