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| Barrella,Karina Medici; Garrafa,Patrícia; Monezi,Telma Alves; Hársi,Charlotte Marianna; Salvi,Cleber; Violante,Paula Alessandra B. Costa; Mehnert,Dolores Ursula. |
| The aim of this study was to verify the presence and annual distribution of adenoviruses and hepatitis A virus in domestic sewage in the city of Limeira, São Paulo. Fifty samples with a volume of 8 liters each were collected weekly from December 2004 to December 2005. The viruses were concentrated by filtration through positively charged ZP60S filter membranes, followed by ultracentrifugation. Human adenoviruses (HAdV) were detected by PCR followed by nested-PCR and screening for species F was done by restriction of the PCR product with TaqI endonuclease. Virus infectivity assays were performed by inoculation of concentrates onto HEp-2 cell monolayers. RT-PCR was used for the detection of hepatitis A virus. HAdV were detected in all samples, and 64% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenoviruses; Human adenovirus F; Hepatitis A virus; Sewage treatment. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100017 |
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