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Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes 79
MAGRO,A. J.; SILVA,R. J. DA; RAMOS,P. R. R.; CHERUBINI,A. L.; HATAYDE,M. R..
The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalus durissus terrificus; Intraspecific variation; Protein composition; Venom; Sex; Feeding; Electrophoresis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000200010
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