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	Provedor de dados 58 (1) Autor Ghori,M. I (1) Hameed,A (1) Iqbal,M. J (1) Palavra-chave Bacillus sp (1) Kinetic study (1) Lipases (1) Organic solvents (1) P-Nitrophenyl laurate (1) Tannery wastes (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2011 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes 58 Ghori,M. I; Iqbal,M. J; Hameed,A. Kinetics of a lipase isolated from Bacillus sp. was studied. The enzyme showed maximum activity at pH 9 and temperature 60ºC. The Michaelis constant (K M 0.31 µM) obtained from three different plots i.e., Lineweaver-Burk, Hanes-Wolf and Hofstee, was found to be lower than already reported lipases that confirmed higher affinity of the enzyme for its substrate p-NPL (p-nitrophenyl laurate). Vmax of the enzyme was found to be 7.6 µM/mL/min. Energy of activation calculated from Arrhenius plot was found to be 20.607 kJmol-1. Activation enthalpy (ΔH*) had negative trend and the value for the hydrolysis of p-NPL by the enzyme at optimum temperature was -2.748 kJmol-1 . Activation entropy (ΔS*) and free energy of activation (ΔG*) of the enzyme were found to be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus sp; Kinetic study; Lipases; P-Nitrophenyl laurate; Tannery wastes; Organic solvents. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100003 Registros recuperados: 1 Primeira ... 1 ... Última

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