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Influence of environmental parameters on Karenia selliformis toxin content in culture ArchiMer
Medhioub, Amel; Medhioub, Walid; Amzil, Zouher; Sibat, Manoella; Bardouil, Michele; Ben Neila, Idriss; Mezghani, Salah; Hamza, Asma; Lassus, Patrick.
Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabes gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae oil enriched Gaillard f/2 medium. The influence of growing conditions oil toxin content was studied, examining the effects of (i) different culture Volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15 degrees C et 20-21 degrees C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 +/- 0.14 div d(-1)) was obtained at 20 degrees C...
Tipo: Text Palavras-chave: Mouse bioassay; Gymnodimine; Toxicity; Growth; Karenia selliformis.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7383.pdf
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