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	Provedor de dados BABT (1) Autor Benati,Fabrício José (1) Hashimoto,Newton (1) Lauretti,Flávio (1) Linhares,Rosa Elisa Carvalho (1) Nozawa,Carlos Mitihiko (1) Palavra-chave Enzyme immunoassay (1) Poliovirus antigen (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2007 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Development of an enzyme immunoassay for poliovirus antigens BABT Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko. An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immunoassay; Poliovirus antigen. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005 Registros recuperados: 1 Primeira ... 1 ... Última

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