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Molecular cloning, characterization and enzymatic properties of a novel βeta-agarase from a marine isolate Psudoalteromonas sp. AG52 BJM
Oh,Chulhong; Nikapitiya,Chamilani; Lee,Youngdeuk; Whang,Ilson; Kang,Do-Hyung; Heo,Soo-Jin; Choi,Young-Ung; Lee,Jehee.
An agar-degrading Pseudoalteromonas sp. AG52 bacterial strain was identified from the red seaweed Gelidium amansii collected from Jeju Island, Korea. A β-agarase gene which has 96.8% nucleotide identity to Aeromonas β-agarase was cloned from this strain, and was designated as agaA. The coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (GHF)-16. The predicted molecular mass of the mature protein was 32 kDa. The recombinant β-agarase (rAgaA) was overexpressed in Escherichia coli and purified as a fusion protein. The optimal temperature and pH for activity were 55 ºC and 5.5, respectively. The enzyme had a specific activity of 105.1 and 79.5 unit/mg toward agar and agarose, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar; Aeromonas sp; Β-agarase; Pseudoalteromonas sp; GHF-16; Neoagaro-oligosaccharides.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400006
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Variation of Spirulina maxima biomass production in different depths of urea-used culture medium BJM
Affan,Md-Abu; Lee,Dae-Won; Al-Harbi,Salim Marzoog; Kim,Han-Jun; Abdulwassi,Najah Ibrahim; Heo,Soo-Jin; Oh,Chulhong; Park,Heung-Sik; Ma,Chae Woo; Lee,Hyeon-Yong; Kang,Do-Hyung.
Fewer studies have assessed the outdoor cultivation of Spirulina maxima compared with S. platensis, although the protein content of S. maxima is higher than S. platensis. Spirulina growth medium requires an increased amount of NaHCO3, Na2CO3, and NaNO3, which increases the production cost. Therefore, the current study used a low-cost but high-efficiency biomass production medium (Medium M-19) after testing 33 different media. The medium depth of 25 cm (group A) was sub-divided into A1 (50% cover with a black curtain (PolyMax, 12 oz ultra-blackout), A2 (25% cover), and A3 (no cover). Similarly the medium depths of 30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively, and the effects of depth and surface light...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spirulina maxima; Fertilizer-grade urea; Medium depth; Surface cover; Outdoor mass culture.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400991
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