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| Tozzini,Alejandro C.; Martínez,M. Carolina; Lucca,M. Florencia; Vázquez Rovere,Cecilia; Distéfano,Ana Julia; del Vas,Mariana; Hopp,Esteban. |
| The detection and exact quantification of the presence of GMOs (genetically modified organisms, also named as living modified organisms, LMOs) grains has become very important in international commercial transactions, especially from countries producing both types of commodities, GMOs and GMO-free. This makes necessary to check every batch previous delivery to the recipient country. Several PCR protocols have been proposed to detect the presence of GMO DNA in a sample due to its sensitivity and independence of environmental and physiological influences. However, most of them are qualitative assays and dont give a good quantitative estimation of the detected signal. We developed a semi-quantitative method based on the comparison of the mass of the... |
| Tipo: Journal article |
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| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200006 |
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| Tosto,Daniela; Hopp,Esteban. |
| Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor that belongs to an alliance of species sharing the same basic chromosome number (x = 8). Nuclear rDNA units of O. tuberosa and a selected group of four related diploid species were characterised by RFLP using different restriction endonucleases and southern hybridization probes to produce a restriction map for EcoRI and BamHI. The major rDNA unit length in O. tuberosa was estimated at 10.7 kbp. As expected, restriction site variation was observed mainly in the intergenic... |
| Tipo: Journal article |
Palavras-chave: Andean crops; Oca; Oxalis; RDNA; RFLPs. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002 |
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