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Shi,Weifeng; Hou,Xueling; Li,Xiang; Peng,Hongjun; Shi,Mei; Jiang,Qingbo; Liu,Xiping; Ji,Yun; Yao,Yuhua; He,Caizhen; Lei,Xiangdong. |
BACKGROUND: Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in response to viral infection. The aim of this study was to explore the function and mechanism of MAPK signaling pathway in enterovirus 71 (EV71) infection of human rhabdomyosarcoma (RD) cells. METHODS: Apoptosis of RD cells was observed using annexin V-FITC/PI binding assay under a fluorescence microscope. Cellular RNA was extracted and transcribed to cDNA. The expressions of 56 genes of MAPK signaling pathway in EV71-infected RD cells at 8 h and 20 h after infection were analyzed by PCR array. The levels of IL-2, IL-4, IL-10, and TNF-α in the supernatant of RD cells infected with EV71 at different time points were measured by ELISA. RESULTS: The viability of RD... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterovirus 71; Rhabdomyosarcoma cells; MAPK; PCR array. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400005 |
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Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling; Peng,Hongjun; Shi,Weifeng. |
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027 |
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