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In vitro plant regeneration via indirect organogenesis from different explants of Lathyrus sativus L. and Lathyrus cicera L. Phyton
Li,RS; Tao,YJ; Liu,FJ; Hu,X; Xu,QL; Li,KY.
The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flatpod peavine; Grass pea; Green-nodular callus; In vitro regeneration.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000100012
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Ectopic expression of the PttKN1 gene in Cardamine hirsuta mediated via the floral dip method Phyton
Chen,XJ; Guo,RJ; Tao,YJ; Hu,X; Xu,QL.
PttKN1 gene (Populus tremula × P. tremuloides KNOT-TED1) was isolated from the vascular cambium of a hybrid aspen. Previous studies on transformed plants with the PttKN1 gene suggested that it plays roles in plant development (typically in meristem initiation), maintenance and organogenesis in simple-leaved species. To investigate the gene functions further, sequence analysis of the deduced amino acid was conducted. The results suggested that the gene belongs to the class I KNOX gene (KNOTTED1-like homeobox genes) family and might play important roles in plant development by coding a transcription factor. The gene was introduced into Cardamine hirsuta using the floral dip method mediated via Agrobacterium tumefaciens. The primary transformed plants were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardamine hirsuta; PttKN1 gene; Floral dip; Cardamine hirsuta; Gen PttKN1; Inmersión floral.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200015
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Phylogenetic and Pathotypic Characterization of a Newcastle Disease Virus Strain Isolated from Ducks and Pigeons in Hubei, China Rev. Bras. Ciênc. Avic.
Wang,Y; Zhang,H; Gu,C; Cheng,G; Zhang,W; Mehmood,K; Hu,X.
ABSTRACT Newcastle disease is a highly contagious disease responsible for major outbreaks and considerable economic losses in the poultry industry in China. There is still little information available regarding gene characterization of the NDV, especially in ducks and pigeons. Therefore, the aim of this study was to investigate NDV isolated from ducks and pigeons in Hubei, China. In this study, three NDVs from ducks and pigeons were isolated between 2013 and 2015.The fusion protein (F) gene of the NDV isolates was sequenced and phylogenetically analyzed. The clinical signs and gross histopathological lesions were examined. Phylogenetic analysis of these strains indicated that all the sequences are classified as genotype II. The isolates shared a 112...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle disease virus; Phylogenetic analysis; Pathology; Ducks; Pigeons..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100145
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