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| Scheffer, K. C.; Fahl, W. O.; Iamamoto, K.; Carnieli Jr., P.; Carrieri, M. L.; Oliveira, R. N.; Ito, F. H.. |
| Molecular techniques have been used increasingly as tools for the diagnosis by detecting the rabies virus genome. This study aimed to detect the presence of rabies virus in the wash of skull and in different organs of the genus Artibeus bats using the hemi-nested RT-PCR (hnRT-PCR) and Real Time RT-PCR molecular techniques. From approximately 4,000 specimens of bats received at the Institute Pasteur for rabies diagnosis, 30 bats of the genus Artibeus were selected, with records of positive results for rabies by the traditional techniques of direct fluorescent antibody test (FAT) and inoculation of murine neuroblastoma cell line (N2A). Salivary glands, urinary bladders, kidneys, lungs, and also the washes of the skullcaps of the specimens were collected. The... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3105 |
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| Carnieli Jr., P.; Batista, H. B.C.R.; Schereffer, K. C.; Fahl, W. O.; Lima, J. Y.O.; Oliveira, R. N.; Castilho, J. G.; Iamamoto, K.; Carrieri, M. L.; Kotait, I.. |
| The identification of animal species that transmit pathogens such as the rabies virus is of the utmost importance for public health and the natural history of infectious and contagious diseases. Diagnostic laboratories very often receive mauled or decomposing animal carcasses, particularly of bats, rendering morphometric identification unviable. The existence of different regional names for the same animal, morphological variability and the lack of staff trained in zoological identification constitute a serious problem for epidemiological surveillance. Molecular techniques are used routinely and effectively in systematics, evolution and ecology to identify species and can even be used to identify hybrids that originated from genetically close animals, in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3106 |
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| Ikeda, M. A. K.; Barros, R. F.; Lima, J. Y. O.; Ferreira, J. S.; Pereira, P. M. C.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| The bats are a major reservoir of rabies virus and have a relevant importance in the disease transmission. The aim of this study was to evaluate the bat population submitted for rabies diagnosis. Data were analyzed from 18.805 bats received by the diagnostic section of the Pasteur Institute of Sao Paulo, originated from several counties of the state of Sao Paulo over the period from 2007 to 2012. These specimens were morphologically classified according to their family, at the time of execution of the rabies diagnosis technique. The central nervous system from these animals was submitted to direct immunofluorescence test and, when viable, to viral isolation. From the total of bats received, 76.92% bats belonged to the Molossidae family, 12.14% to the... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17383 |
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| Pereira, P. M. C.; Lima, J. Y. O.; Barros, R. F.; Ferreira, J. S.; Ikeda, M. A. K.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Asano, K. M.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| Bats found in Brazil are classified in 9 families, 64 genus and 168 species. They are an important rabies reservoirs and all species can be infected or be the transmitter of rabies virus, independently of their specie classification or feeding habits. This work aimed a survey of bats species originated from counties of Sao Paulo state, which were sent to the diagnostic laboratory of the Pasteur Institute – Sao Paulo. Between August 2009 to October 2012; 9.109 bats specimens were received and submitted to recommended techniques for rabies diagnosis by World Health Organization. From these specimens, 8.421 (92,45%) were classified in family, genus and specie, by morphological and morphometric parameters using bats identification keys. Furthermore, they also... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17386 |
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