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Caracterização parcial e utilização da fitase extraída de sementes germinadas de girassol PAB
Agostini,Juliana da Silva; Ida,Elza Iouko.
O objetivo deste trabalho foi ativar, extrair e caracterizar parcialmente a fitase em sementes germinadas de girassol (Helianthus annuus L.), híbrido M734, e avaliar o efeito da fitase no farelo de girassol. Sementes foram colocadas para germinar por oito dias em câmara a 25°C. A fitase foi extraída com CaCl2 2%, depois do quinto dia de germinação, e fracionada com (NH4)2SO4 até 80% de saturação. O extrato bruto foi caracterizado parcialmente e aplicado em farelo de girassol desengordurado para avaliar a hidrólise do fitato. Com a germinação, houve aumento na atividade da fitase e redução no teor de fitato. A maior atividade da fitase foi observada do quinto ao oitavo dia de germinação. A fitase das sementes germinadas aos cinco dias apresentou atividade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helianthus annuus; Girassol híbrido; Hidrólise de fitato.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600021
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Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh PAB
Borges,Cristiane Wing Chong; Carrão-Panizzi,Mercedes Concórdia; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Benedetti,Silvia; Ida,Elza Iouko.
Abstract: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β-glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Rhizopus oligosporus; Daidzein; Fermented soybean; Functional food; Genistein.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000300271
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Efeito da germinação de soja CV. BR-13 e Paraná sobre ácido fítico, fósforo total e inibidores de tripsina PAB
Ribeiro,Mara Lucia Luiz; Ida,Elza Iouko; Oliveira,Maria Cristina Neves de.
Este trabalho foi conduzido para avaliar o efeito da germinação sobre os constituintes antinutricionais da soja, ácido fítico, P total e inibidores de tripsina, que interferem na utilização de proteínas e outras substâncias. A diminuição no aproveitamento de nutrientes resulta em inibição de crescimento, hipoglicemia ou danos a tecidos animais. Para os ensaios foram utilizadas as cultivares de soja (Glycine max (L.) Merrill ) BR-13 e Paraná. As sementes foram incubadas em câmara de germinação a 25°C e 100% de umidade, de 0 a 72 horas, com coleta de amostras em intervalos eqüidistantes de seis horas. A cultivar Paraná apresentou teores superiores de ácido fítico, P total e inibidores de tripsina, em relação à cultivar BR-13. As análises de variância e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Constituintes antinutricionais.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000100005
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Changes in the isoflavone profile and in the chemical composition of tempeh during processing and refrigeration PAB
Ferreira,Márcia Pires; Oliveira,Maria Cristina Neves de; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Carrão-Panizzi,Mercedes Concórdia.
The objective of this work was to analyze changes in the isoflavone profile, determined by high performance liquid chromatography, at different processing stages and after refrigeration of tempeh. For tempeh production, clean soybean grains from cultivars BR 36 (low isoflavone content) and IAS 5 (high) were dehulled, and the separated cotyledons were hydrated and then cooked in boiling water for 30 min. Spores of the fungus Rhizopus microsporus var. oligosporus were inoculated in the cooked and cooled cotyledons, and incubated at 32ºC for 6, 12, 18, and 24 hours in perforated polypropylene bags, for fermentation. The resulting tempeh was stored at 4ºC for 6, 12, 18, and 24 hours. After 24-hour fermentation, isoflavone glucosides were 50% reduced, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizopus microsporus var. oligosporus; Aglycone; Fermentation; Functional food; High performance liquid chromatography; Malonyl.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100018
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Physicochemical characteristics of canned vegetable-type soybean processed with zinc at different pasteurization times PAB
Czaikoski,Karina; Leite,Rodrigo Santos; Mandarino,José Marcos Gontijo; Carrão-Panizzi,Mercedes Concórdia; Silva,Josemeyre Bonifácio Da; Ida,Elza Iouko.
Abstract: The objective of this work was to evaluate the effects of zinc concentration in acidified brine and of pasteurization time on the zinc content, color, and physicochemical and microbiological characteristics of canned vegetable-type soybean (Glycine max). The 22 central composite rotational design (CCRD) was used, with zinc concentration in the acidified brine and duration of pasteurization as variables, resulting in 12 assays. A canning formulation was developed and compared with canned-vegetable type soybean without zinc and with fresh-vegetable type soybean, with microbiological evaluation. Zinc addition within the limits defined by Food and Drug Administration (≤75 ppm) does not affect the physicochemical and microbiological characteristics of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Edamame; Isoflavones; Oligossacharides; Thermal processing.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700840
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Germination conditions influence the physical characteristics, isoflavones, and vitamin C of soybean sprouts PAB
Silva,Mariah Benine Ramos; Leite,Rodrigo Santos; Oliveira,Marcelo Álvares de; Ida,Elza Iouko.
Abstract: The objective of this work was to evaluate the effects of the germination conditions of 'BRS 216' soybean (Glycine max) on the length, yield, and isoflavone contents of sprouts. A 23 factorial arrangement was used to evaluate the variables soaking time, irrigation frequency, and germination time. Sprouts that showed better length and yield and higher isoflavone contents were evaluated for their chemical composition and vitamin C content. Soaking and germination time of soybean showed a positive and significant linear effect on sprout length. However, only germination time showed a significant positive linear effect on yield, and a significant negative linear effect on the content of malonylglycosides, aglycones, and total isoflavones. Soybean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Aglycones; Ascorbic acid; Proximate composition; Soaking.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2020000104000
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Cinética de inativação de inibidores de tripsina e de insolubilização de proteínas de diferentes cultivares de soja R. Bras. Zootec.
Miura,Edna Mayumi Yuahasi; Silva,Rui Sérgio dos Santos Ferreira da; Mizubuti,Ivone Yurika; Ida,Elza Iouko.
A soja (Glycine max (L.) Merrill) é uma das leguminosas mais estudadas, em virtude de sua intensa utilização como fonte de óleo comestível e proteína para alimentação humana e animal. A presença de inibidores de tripsina na soja limita sua utilização, tornando necessário o processamento térmico para melhorar sua qualidade nutricional. Entretanto, o processamento térmico excessivo ou inadequado pode reduzir a qualidade da proteína. Neste trabalho, foram utilizados cultivares de soja BR-36, com atividade normal de inibidores de tripsina, e cultivares BRM 95-5297 e BRM 95-5262, com baixas atividades de inibidores de tripsina. As farinhas com 10 e 16% de umidade foram aquecidas em banho a 95°C, com cinco intervalos (15, 25, 50, 75 e 100 minutos) de retenção;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatores antinutricionais; Insolubilidade da proteína; Parâmetros cinéticos; Tempo de retenção; Tratamento térmico.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000500028
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Avaliação biológica de linhagem de soja com baixa atividade de inibidores de tripsina R. Bras. Zootec.
Miura,Edna Mayumi Yuahasi; Binotti,Marco Aurélio R.; Camargo,Daliane Souza de; Mizubuti,Ivone Yurika; Ida,Elza Iouko.
Cultivar de soja BR 36 com atividades de inibidores de tripsina convencional e linhagem de soja BRM 95-5262 geneticamente modificadas para conter baixas atividades de inibidores de tripsina foram utilizados em ensaios biológicos com aves contendo 40 e 20% e 30 e 20% de atividades residuais relativa de inibidores de tripsina, respectivamente. Os valores médios de PER, NPR e CA indicaram que os tratamentos de soja crua foram inferiores aos tratamentos de soja processados termicamente. Entretanto, tratamentos de soja processados termicamente com inativação de 20% de atividades residuais relativa de inibidores de tripsina não apresentaram diferenças significativas entre si e em relação ao padrão farelo de soja. Porém, quando as atividades de inibidores de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aves; Ensaio biológico; Inbidores de tripsina; Soja.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000600023
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Rates of calcium, yield and quality of snap bean Scientia Agricola
Favaro,Simone Palma; Braga Neto,José Antônio; Takahashi,Hideaki Wilson; Miglioranza,Édison; Ida,Elza Iouko.
Calcium ions (Ca) play an important role in many biochemical processes, delaying senescence and controlling physiological disorders in fruits and vegetables. The objective of this experiment was to analyze the effect of increasing calcium concentrations in snap beans. Snap bean cultivar UEL 1 was sown in sand containing 80 mg L-1 of calcium supplemented with nutrient solution, plus calcium at different contents: 0, 75, 150 and 300 mg L-1. Ca was mainly recovered in the shoots, followed by roots and pods. Calcium concentrations in the pods were 130, 259, 349 and 515 mg 100 g-1dry matter on a dry weight basis, in relation to the enhancement of calcium contents in the nutrient solution, respectively. A negative relationship between nitrogen content in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Biomass production; Pod; Nutrient solution; Nitrogen.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600009
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Cor de feijão vagem fresco e processado após aplicação de cálcio Scientia Agricola
Fávaro,Simone Palma; Sá,Marcela Colognesi de; Ida,Elza Iouko; Braga Neto,José Antônio.
A cor em vegetais é um dos principais atributos de qualidade observado pelo consumidor e é influenciada por características intrínsecas e de processamento. O objetivo desta investigação foi avaliar as alterações de cor de feijão vagem fresco e processado após aplicação de concentrações crescentes de cálcio via absorção radicular. O feijão vagem cultivar UEL 1 foi cultivado com aplicação de concentrações crescentes de cálcio via solução nutritiva contendo 0, 75 , 150 e 300 mL L-1 de cálcio em substrato de areia grossa. A cor das vagens ao natural e enlatadas foram avaliadas por colorimetria de três estímulos, e os resultados foram expressos no sistema Hunter. As vagens ao natural apresentaram-se mais claras e amarelas quando foi adicionado cálcio à solução...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Phaseolus vulgaris; Feijão-vagem; Cor; Cálcio.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300030
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Influence of Cooling on the Glycolysis Rate and Development of PSE (Pale, Soft, Exudative) Meat BABT
Pedrão,Mayka Reghiany; Kato,Talita; Soares,Adriana Lourenço; Ida,Elza Iouko; Coró,Fábio Augusto Garcia; Grespan,Moises; Paião,Fernanda; Shimokomaki,Massami.
The aim of this work was to evaluate pH values fall rate in chicken breast meat under commercial refrigeration processing conditions and the development of PSE (pale, soft, exudative) meat. Broiler breast samples from the Cobb breed, both genders, at 47 days of age (n = 100) were taken from refrigerated carcasses (RS) immersed in water and ice in a tank chilled at 0°C (±2). pH and temperature (T) values were recorded at several periods throughout refrigeration in comparison to samples left at room T as control (CS). The ultimate pH (pHu) value of 5.86 for RS carcasses were only reached at 11°C after 8.35 h post mortem (PM) while, for CS samples, pHu value was 5.94 at 22°C after 4.08 h PM. Thus, under commercial refrigeration conditions, the glycolysis rate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Post mortem management; Commercial processing refrigeration; Water chiller.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200272
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Soybean β-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the aglycones BABT
Grade,Luciana Carvalho; Moreira,Amanda Aleixo; Varea,Geni da Silva; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Ribeiro,Mara Lúcia Luiz.
The objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 23 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-glucosidase soybean; Immobilisation; Chitosan; Aglycones.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500766
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Effects of storage time and temperature on the characteristics of vegetable-type soybean grain minimally processed BABT
Czaikoski,Karina; Carrao-Panizzi,Mercedes Concordia; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko.
The objective of this study was to evaluate the effects of storage time and temperature on the characteristics of vegetable-type soybean grain (cultivar BRS 267) minimally processed and to define the best conditions for its storage. The evaluation was performed by measurement of vitamin C levels, weight loss and color parameters (L*, a*, and b*). The time of storage of vegetable-type soybean grains minimally processed and storage in Styrofoam trays and wrapped with PVC film, caused a decreased in vitamin C levels and color parameters and increased weight loss. This process was intensified with higher temperature at 25 °C than 5°C. To maintain appropriate levels of vitamin C, weight and color of vegetable-type soybean grains minimally processed and storage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vegetable-type soybean; Quality; Edamame.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400002
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Broiler chicken PSE (Pale, Soft, Exudative) meat and water release during chicken carcass thawing and brazilian legislation BABT
Kato,Talita; Barbosa,Claudia Freitas; Ida,Elza Iouko; Soares,Adriana Lourenço; Shimokomaki,Massami; Pedrao,Mayka Reghiany.
The objective of this work was to investigate the relationship between poultry PSE meat and water loss of frozen chicken carcass in two experiments. The first experiment was carried out in commercial abattoir. Poultry carcass were classified as PSE meat (n=59) (pH <5.8) and control samples (n=55) (pH &gt;5.8). Water absorption and drip test were performed according to the Brazilian legislation methodologies. The second experiment was carried out with commercial whole five brands frozen carcasses purchased from the local supermarkets (n=30) and analyzed for pH, water holding capacity (WHC) and drip test. PSE poultry meats absorbed 3.59% of water during the processing similar to the control samples; however, released 0.38% more water during thawing of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drip loss; Water absorption; Water holding capacity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600015
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Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer BABT
Oda,Sandra Helena Inoue; Nepomuceno,Alexandre Lima; Ledur,Mônica Corrêa; Oliveira,Maria Cristina Neves de; Marin,Silvana Regina Rockenbach; Ida,Elza Iouko; Shimokomaki,Massami.
Total RNA isolated from Pectoralis major muscle from PSE (L*24h>53.0, pH<5.8) and non-PSE (44<"L*24h>"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p<0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p>0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malignant hyperthermia; Poultry; Gene expression; Real time PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600024
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Sensitivity to halothane and its relationship to the development of PSE (Pale, Soft, Exudative) meat in female lineage broilers BABT
Marchi,Denis Fabrício; Trindade,Marco Antônio; Oba,Alexandre; Soares,Adriana Lourenço; Ida,Elza Iouko; Ferraz,José Bento Sterman; Michelan Filho,Tércio; Ziober,Iris Lamberti; Shimokomaki,Massami.
This work aimed to evaluate female lineage broilers for halothane sensitivity and for their susceptibility to the subsequent development of PSE meat. The halothane test was carried out in an anesthetic chamber with 3.0% halothane. The unconscious birds were examined for leg muscle rigidity. If one or both legs became extended and rigid, the birds were classified as halothane sensitive (HAL+), while unresponsive birds were classified as halothane negative (HAL-). The results showed that of 298 birds aged 42 days old, 95.6% were HAL- and 4.4% were HAL+. A sample of pectoralis major muscle was collected from HAL- (n=105) and HAL+ (n=13) birds. The pH and breast fillet color were determined at 4ºC, 24 hours post-mortem. Interestingly, only 2.5% of HAL+ birds...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environmental factors; Pectoralis major; Meat color abnormality.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700028
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Vehicle thermal microclimate evaluation during Brazilian summer broiler transport and the occurrence of PSE (Pale, Soft, Exudative) meat BABT
Simões,Gislaine Silveira; Oba,Alexandre; Matsuo,Tiemi; Rossa,Alessandro; Shimokomaki,Massami; Ida,Elza Iouko.
The formation of a thermal microclimate within the vehicle during the transport of broilers from farm to slaughterhouse affects the birds' welfare and potentially promotes the development of PSE meat. Thus, the aim of this work was to evaluate the effects of routine commercial practices on the vehicle microclimate formed during transportation of broilers. Twenty-four hours postmortem Pectoralis major m. samples were classified as PSE meat by determining pH and color (L*, a* e b*). Results showed that broiler located at the rear of the truck and with longer journey presented higher amounts of PSE meat because birds were under harsh conditions of both temperature and relative humidity. The ventilation decreased gradually from the front to the rear of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal welfare; Poultry meat quality; Thermal stress; Poultry breast meat; Color abnormality.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700025
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The effect of management of transport and lairage conditions on broiler chicken breast meat quality and DOA (Death on Arrival) BABT
Oba,Alexandre; Almeida,Mauricio de; Pinheiro,João Waine; Ida,Elza Iouko; Marchi,Denis Fabricio; Soares,Adriana Lourenço; Shimokomaki,Massami.
The aim of this work was to evaluate the influence of time of broiler chicken transportation and lairage prior to slaughtering on the occurrence of PSE (Pale, Soft, Exudative) meat and Death On Arrival (DOA) under non-commercial conditions in the Brazilian summer. Male birds (n=250) from a commercial line were subjected to different periods of journey (30, 90, and 180 min) and lairage (0, 90, and 180 min) before slaughtering. The occurrence of PSE was higher in broilers subjected to shorter journeys and lairage periods, whereas DOA was more pronounced upon longer periods of transport and lairage. The DOA occurrence percentage was much higher in comparison to commercially available figures, confirming that broiler chickens are very sensitive to both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water holding capacity; PSE meat; Color abnormalities; Broiler management.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700026
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Lowering of phytic acid content by enhancement of phytase and acid phosphatase activities during sunflower germination BABT
Agostini,Juliana da Silva; Nogueira,Rosicler Balduíno; Ida,Elza Iouko.
The objective of this work was to investigate the germination of hybrid sunflowers BRS191 and C11 as a means of lowering phytic acid (PA) content by enhancing the activity of endogenous phytase and acid phosphatase. The concentration of PA in hybrid sunflower achenes varied from 2.16 to 2.83g/100g of sample (p < 0.05). The phytase and acid phosphatase activities of sunflowers BRS191 and C11 were the highest on the 4th and 5th days of germination, respectively, with the release of the phosphorus. These results indicated that hybrid sunflower PA reduced and enhance phytase activity at distinct germination periods, which could open up the possibility of applying these enzymes in the control of PA content in cereals, thus improving their nutritional value.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Germination; Sunflower hybrids; Phytic acid; Phytase; Acid phosphatase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400028
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Evaluation of the shelf-life of vegetable-type soybean pods BABT
Santana,Andréia Cristina; Carrao-Panizzi,Mercedes Concordia; Mandarino,José Marcos Gontijo; Leite,Rodrigo Santos; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko.
The shelf-life of the vegetable-type soybean pods stored under different conditions was evaluated by chemical characteristics and color. The pods were harvested in the R6 stage and stored either at 30 or 7ºC for 9 d. After the storage period, the pods were blanched and threshed, and the immature green grains were used for the analysis. The protein content decreased after 6 d of storage at 7ºC. There was no difference in the lipid content after the storage at 30 and 7ºC for 9 d. The starch and sucrose contents decreased after the first day of storage at 30ºC. There was no difference in trypsin inhibitor activity until 6 d of storage at 30 and 7ºC. The green color of the pods that was an indication of the quality that was maintained when stored at 7ºC during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vegetable-type soybean; Sucrose; Starch; Trypsin inhibitor; Color.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400015
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