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| Carvalho,Patrícia de Oliveira; Contesini,Fabiano Jares; Ikegaki,Masaharu. |
| The enantioselectivity (E) of native lipases from Aspergillus niger, Aspergillus terreus, Fusarium oxysporum, Mucor javanicus, Penicillium solitum and Rhizopus javanicus in the resolution of (R,S)-ibuprofen and (R,S)-ketoprofenenantiomers by esterification reaction with 1-propanol in isooctane was compared with known commercial Candida rugosa (Sigma) and Candida antarctica (Novozym®435) lipases. In the resolution of (R,S)-ibuprofen, C. rugosa lipase showed good selectivity (E = 12) while Novozym®435 (E = 6.7) and A. niger (E = 4.8) lipases had intermediate selectivities. Other enzymes were much less selective (E around 2.3 and 1.5), under tested conditions. After preliminary optimization of reaction conditions (water content, enzyme concentration and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipase (R; S)-ibuprofen (R; S)-ketoprofen enantioselectivity. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300024 |
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| Fernandes,Maurette dos Reis Vieira; Silva,Tales Alexandre Costa e; Pfenning,Ludwig Heinrich; Costa-Neto,Cláudio Miguel da; Heinrich,Tassiela Andréa; Alencar,Severino Matias de; Lima,Marisa Aparecida de; Ikegaki,Masaharu. |
| A total of 22 endophytic fungi isolated from coffee (Coffea arabica L.) were cultivated in vitro and their crude extracts tested. The screening was carried out using the agar diffusion method against Staphylococcus aureus, Escherichia coli and Candida albicans. The most effective isolate was Alternaria alternata, and subsequently, its extract was assayed. The total phenolic content was 3.44 μg GAE/mg of the crude extract. For the antibacterial and antifungal activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal and fungicidal concentrations (MBC and MFC) were determined. The ranges of MIC values were 50-100 μg/mL for S. aureus and 400-800 μg/mL for E. coli. The extract did not show activity in the tested concentrations for C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endophytic fungi; Bioactive compounds; Alternaria alternata/extract/antioxidant activity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400010 |
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