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Diagnosis and quantitative detection of HSV DNA in samples from patients with suspected herpes simplex encephalitis BJID
Ziyaeyan,Mazyar; Alborzi,Abdolvahab; Haghighi,Afshin Borhani; Jamalidoust,Marziyeh; Moeini,Mahsa; Pourabbas,Bahman.
Diagnosis of herpes simplex encephalitis (HSE) is based on the detection of herpes simplex virus (HSV) DNA in patients' CSF samples. HSV DNA quantitation has the potential for estimating the effects of antiviral therapy. The aim of this study was to diagnose HSV DNA in HSE suspected patients and the quantitative analysis of its genome using real-time PCR to assess the value of the viral load in the course of antiviral treatment. The CSF samples were collected from 236 consecutive HSE suspected patients from November 2004 to May 2008. Upon DNA extraction, the samples were analyzed by Real-Time PCR assay. A set of primers amplified a common sequence of HSV glycoprotein B gene. The copy numbers of unknown samples were expressed via a standard curve drawn with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encephalitis; Herpes simplex; Viral load; Acyclovir; Quantitative analysis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300005
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