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	Provedor de dados BJM (1) Autor Fernández,Bárbara (1) Gilardoni,Liliana Rosa (1) Jar,Ana María (1) Mendez,Laura (1) Morsella,Claudia (1) Mundo,Silvia Leonor (1) Paolicchi,Fernando Alberto (1) Mais... Palavra-chave Cow's milk (1) Immunomagnetic separation (1) Mycobacterium avium subsp. paratuberculosis (1) PCR (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2016 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR BJM Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor. Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506 Registros recuperados: 1 Primeira ... 1 ... Última

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