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| Lédo,Ana da Silva; Jenderek,Maria; Skogerboe,Dianne; Staats,Elise; Machado,Caroline de Araújo; Oliveira,Leila Albuquerque Resende de. |
| Abstract: The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocos nucifera; Ex situ conservation; PVS3. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000400514 |
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