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	Provedor de dados BJMBR (2) Autor Jiang,L. (2) Liang,Y. (1) Wang,G. (1) Wang,K. (1) Wei,X. (1) Xiao,X. (1) Yang,N. (1) Yang,P. (1) Yang,T. (1) Zhang,B. (1) Zuo,X. (1) Mais... Palavra-chave Cell apoptosis (1) Cell viability (1) Expression (1) Lung cancer (1) MicroRNA-486 (1) Mipu1 (1) Polyclonal antibody (1) Propofol (1) Purification (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Propofol inhibits lung cancer cell viability and induces cell apoptosis by upregulating microRNA-486 expression BJMBR Yang,N.; Liang,Y.; Yang,P.; Yang,T.; Jiang,L.. Propofol is a frequently used intravenous anesthetic agent. Recent studies show that propofol exerts a number of non-anesthetic effects. The present study aimed to investigate the effects of propofol on lung cancer cell lines H1299 and H1792 and functional role of microRNA (miR)-486 in these effects. H1299 and/or H1792 cells were treated with or without propofol and transfected or not with miR-486 inhibitor, and then cell viability and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry. The expression of miR-486 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) with or without propofol treatment. Western blot was performed to analyze the protein expression of Forkhead... Tipo: Info:eu-repo/semantics/article Palavras-chave: Propofol; Lung cancer; Cell viability; Cell apoptosis; MicroRNA-486. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100601 Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene BJMBR Wang,G.; Zuo,X.; Jiang,L.; Wang,K.; Wei,X.; Zhang,B.; Xiao,X.. Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1), a novel zinc finger protein, was originally cloned using bioinformatic analysis and 5' RACE technology of rat heart after a transient myocardial ischemia/reperfusion procedure in our laboratory. In order to investigate the functions of Mipu1, the recombinant prokaryotic expression vector pQE31-Mipu1 was constructed and transformed into Escherichia coli M15(pREP4), and Mipu1-6His fusion protein was expressed and purified. The identity of the purified protein was confirmed by mass spectrometry. The molecular mass of the Mipu1 protein was 70.03779 kDa. The fusion protein was intracutaneously injected to immunize New Zealand rabbits to produce a polyclonal antibody. The antibody titer was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mipu1; Purification; Expression; Polyclonal antibody. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100007 Registros recuperados: 2 Primeira ... 1 ... Última

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