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Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae Electron. J. Biotechnol.
Zhang,Min; Shi,Jun; Jiang,Li.
Background Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains...
Tipo: Journal article Palavras-chave: Bioethanol; Random amplified polymorphic DNA (RAPD); Yeast.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300010
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Construction and application of a built-in dual luciferase reporter for microRNA functional analysis Electron. J. Biotechnol.
Bi,Yanzhen; Zheng,Xinmin; Shao,Changwei; Pan,Wen; Jiang,Li; Ouyang,Huiwu.
Background: As key gene regulators, microRNAs post-transcriptionally modulate gene expression via binding to partially complementary sequence in the 3? UTR of target mRNA. An accurate, rapid and quantitative tool for sensing and validation of miRNA targets is of crucial significance to decipher the functional implications of miRNAs in cellular pathways. Results: Taking advantage of an improved restriction-free cloning method, we engineered a novel built-in dual luciferase reporter plasmid where Firefly and Renilla luciferase genes were assembled in a single plasmid named ?pFila?. This design eliminates the transfection of a separate control plasmid and thus minimizes the time and labor required for miRNA-target sensing assays. pFila consistently produces...
Tipo: Journal article Palavras-chave: Biosensor; Luciferase; Ligase-independent; MiRNA; Target.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200011
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