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| Shi,Weifeng; Hou,Xueling; Li,Xiang; Peng,Hongjun; Shi,Mei; Jiang,Qingbo; Liu,Xiping; Ji,Yun; Yao,Yuhua; He,Caizhen; Lei,Xiangdong. |
| BACKGROUND: Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in response to viral infection. The aim of this study was to explore the function and mechanism of MAPK signaling pathway in enterovirus 71 (EV71) infection of human rhabdomyosarcoma (RD) cells. METHODS: Apoptosis of RD cells was observed using annexin V-FITC/PI binding assay under a fluorescence microscope. Cellular RNA was extracted and transcribed to cDNA. The expressions of 56 genes of MAPK signaling pathway in EV71-infected RD cells at 8 h and 20 h after infection were analyzed by PCR array. The levels of IL-2, IL-4, IL-10, and TNF-α in the supernatant of RD cells infected with EV71 at different time points were measured by ELISA. RESULTS: The viability of RD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterovirus 71; Rhabdomyosarcoma cells; MAPK; PCR array. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400005 |
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