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| Juan,Y.H; Chen,Z.H; Chen,L.J; Wu,Z.J; Wang,R; Sun,W.T; Zhang,Y.L. |
| The kinetic characteristics of soil urease have attracted great attention, but little information was available on its kinetic and thermodynamic behaviors as affected by urease inhibitors and temperature. With black soil (Pachic Udic Mollisol) in Heilongjiang Province of Northeast China as a test soil, an incubation test was conducted to investigate the effect of urease inhibitors (hydroquinone, HQ; phenyl phosphorodiamidate, PPD; N-(n-Butyl) thiophosphoric triamide, NBPT) on kinetic and thermodynamic behaviors of soil urease at 10°C, 20°C and 30°C. The results showed that all tested inhibitors increased Km and decreased Vmax, behaving as mixed inhibitors to soil urease. With increasing temperature, the Km and Vmax values increased significantly. With... |
| Tipo: Journal article |
Palavras-chave: Meterkinetic paras; Thermodynamic parameters; Soil urease; Urease inhibitors; Temperatures. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912010000100001 |
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| Juan,Y.H; Chen,L.J; Wu,Z.J; Wang,R. |
| With black soil (Pachic Udic Argiboroll) of Northeastern China as the test object, an incubation test was conducted to investigate the effects of urease inhibitors, hydroquinone (HQ), phenyl phosphorodiamidate (PPD) and N-(n-Butyl) thiophosphoric triamide (NBPT), on the kinetic characteristics of soil urease under normal moisture and waterlogged conditions, aimed to study the changes of catalytic potential of soil urease and the inhibition mechanisms. The results showed that test urease exhibited typical Michaelis-Menten kinetic behaviors, and all test inhibitors increased the Km and decreased the Vmax of soil urease, behaving as mixed inhibitors to soil urease. Under both normal and waterlogged conditions, compared with HQ, PPD and NBPT made Km increase... |
| Tipo: Journal article |
Palavras-chave: Kinetic parameters; Mixed inhibition; Moisture regime; Soil urease. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912009000200004 |
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