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| Juhel, Jean-baptiste; Utama, Rizkie S.; Marques, Virginie; Vimono, Indra B.; Sugeha, Hagi Yulia; Kadarusman,; Pouyaud, Laurent; Dejean, Tony; Mouillot, David; Hocdé, Régis. |
| Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species... |
| Tipo: Text |
Palavras-chave: EDNA metabarcoding; Sequence clustering; Operational Taxonomic Unit; Diversity assessment; Detectability. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00640/75232/79601.pdf |
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| Kadarusman,; Sugeha, Hagi Yulia; Pouyaud, Laurent; Hocdé, Régis; Hismayasari, Intanurfemi B.; Gunaisah, Endang; Widiarto, Santoso B.; Arafat, Gulam; Widyasari, Ferliana; Mouillot, David; Paradis, Emmanuel. |
| Coelacanth fishes of the genus Latimeria are the only surviving representatives of a basal lineage of vertebrates that originated more than 400 million years ago. Yet, much remains to be unveiled about the diversity and evolutionary history of these ‘living fossils’ using new molecular data, including the possibility of ‘cryptic’ species or unknown lineages. Here, we report the discovery of a new specimen in eastern Indonesia allegedly belonging to the species L. menadoensis. Although this specimen was found about 750 km from the known geographical distribution of the species, we found that the molecular divergence between this specimen and others of L. menadoensis was great: 1.8% compared to 0.04% among individuals of L. chalumnae, the other living... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00603/71466/69921.pdf |
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| Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas. |
| DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications... |
| Tipo: Text |
Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf |
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