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Krauss, Maike; Krause, Hans-Martin; Spangler, Simone; Kandeler, Ellen; Behrens, Sebastian; Kappler, Andreas; Mäder, Paul; Gattinger, Andreas. |
Since the development of effective N2O mitigation options is a key challenge for future agricultural practice, we studied the interactive effect of tillage systems on fertilizer-derived N2O emissions and the abundance of microbial communities involved in N2O production and reduction. Soil samples from 0–10 cm and 10–20 cm depth of reduced tillage and ploughed plots were incubated with dairy slurry (SL) and manure compost (MC) in comparison with calcium ammonium nitrate (CAN) and an unfertilized control (ZERO) for 42 days. N2O and CO2 fluxes, ammonium, nitrate, dissolved organic C, and functional gene abundances (16S rRNA gene, nirK, nirS, nosZ, bacterial and archaeal amoA) were regularly monitored. Averaged across all soil samples, N2O emissions decreased... |
Tipo: Journal paper |
Palavras-chave: Soil quality; Soil tillage; Air and water emissions. |
Ano: 2016 |
URL: http://orgprints.org/31140/1/Krauss-Krause-etal-2016-online_BFS.pdf |
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Mosimann, Carla; Oberhänsli, Thomas; Ziegler, Dominik; Nassal, Dinah; Kandeler, Ellen; Boller, Thomas; Mäder, Paul; Thonar, Cécile. |
TaqMan-based quantitative PCR (qPCR) assays were developed to study the persistence of two well-characterized strains of plant growth-promoting rhizobacteria (PGPR), Pseudomonas fluorescens Pf153 and Pseudomonas sp. DSMZ 13134, in the root and rhizoplane of inoculated maize plants. This was performed in pot experiments with three contrasting field soils (Buus, Le Caron and DOK-M). Potential cross-reactivity of the qPCR assays was assessed with indigenous Pseudomonas and related bacterial species, which had been isolated from the rhizoplane of maize roots grown in the three soils and then characterized by Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). Sensitivity of the qPCR expressed as detection limit of... |
Tipo: Journal paper |
Palavras-chave: Soil biology Crop health; Quality; Protection. |
Ano: 2017 |
URL: http://orgprints.org/31294/1/mosimann-etal-2017-fmicb-vol7-article02150.pdf |
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