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	Provedor de dados ArchiMer (1) Autor Choi, Kwang-sik (1) Jeung, Hee-do (1) Kang, Do-hyung (1) Le Moullac, Gilles (1) Park, Heung-sik (1) Palavra-chave Black-lip pearl oyster (1) ELISA (1) Pinctada margaritifera (1) Polyclonal antibody (1) Quantification of eggs and sperm (1) Tipo do documento Text (1) Ano 2014 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) ArchiMer Jeung, Hee-do; Kang, Do-hyung; Park, Heung-sik; Le Moullac, Gilles; Choi, Kwang-sik. We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to... Tipo: Text Palavras-chave: Black-lip pearl oyster; ELISA; Pinctada margaritifera; Polyclonal antibody; Quantification of eggs and sperm. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28659/32145.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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